- Open Access
First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease
© The Author(s). 2016
- Received: 6 July 2016
- Accepted: 26 August 2016
- Published: 13 September 2016
Fanconi anemia (FA) is a chromosomal instability syndrome characterized by increased frequency of chromosomal breakages, chromosomal radial figures and accelerated telomere shortening. In this work we performed detailed molecular-cytogenetic characterization of breakpoints in primary lymphocytes of FA-D2 patients in different stages of the disease using fluorescent in situ hybridization.
We found that chromosomal breakpoints co-localize on the molecular level with common fragile sites, whereas their distribution pattern depends on the severity of the disease. Telomere quantitative fluorescent in situ hybridization revealed that telomere fusions and radial figures, especially radials which involve telomere sequences are the consequence of critically shortened telomeres that increase with the disease progression and could be considered as a predictive parameter during the course of the disease. Sex chromosomes in FA cells are also involved in radial formation indicating that specific X chromosome regions share homology with autosomes and also could serve as repair templates in resolving DNA damage.
FA-D2 chromosomal breakpoints co-localize with common fragile sites, but their distribution pattern depends on the disease stage. Telomere fusions and radials figures which involve telomere sequences are the consequence of shortened telomeres, increase with disease progression and could be of predictive value.
- Fanconi anemia
- Fragile sites
- Telomere fusions
- Radial figures
- X chromosome
Fanconi anemia (FA) is a rare, inherited, genetically heterogeneous chromosomal instability syndrome. To date, 19 different complementation groups, which correspond to distinct DNA repair genes have been identified, FA-A, FA-B, FA-C, FA-D1 (BRCA2), FA-D2, FA-E, FA-F, FA-G, FA-I, FA-J (BRIP1), FA-M, FA-N (PALB2), FA-O (RAD51C), FA-P (SLX4), FA-Q (ERCC4), FA-S (BRCA1), FA-R (RAD51) and FA-T (UBE2T) . The genes that correspond to different FA complementation groups are involved in the FA/BRCA DNA damage repair pathway having an essential function in the cellular response to stress induced by DNA alkylating agents . The most frequent complementation group in Serbia is FA-D2 , which is in contrast to the FA-D2 frequency in the general population where it accounts for approximately 4 % of all complementation groups . FA-D2 patients are characterized by a broad spectrum of congenital abnormalities and, unlike most of the other FA complementation groups, an early onset of hematological manifestations including acute myelogenous leukemia and bone marrow failure . One of the most significant FA cellular characteristics is an increase of chromosomal aberrations induced by interstrand cross-linking (ICL) agents . When exposed to these agents, FA cells show an increased frequency of chromosomal breakages and chromosomal radial figures, prolonged cell-cycle arrest in G2/M phase, reduced cellular survival and accelerated telomere shortening . With respect to chromosomal breakages, Schoder and coworkers  reported that chromosomal breakpoints in cells derived from FA-A and FA-C patients co-localize with the chromosomal regions that are constitutionally prone to breakage in each individual, known as common fragile sites (CFSs). In addition to standard FS classification, it was shown that cells exposed to replication stress also display breakages in telomeric regions; therefore they were attributed to CFSs . Recent studies of telomere maintenance in FA cells showed that telomere fusions arise either as a consequence of their critical shortening or altered capping function. High percentage of dysfunctional telomeres and marked telomere fragility was found to be typical for FA-D2 cellular phenotype . However, the role of telomeres in formation of induced chromosomal aberrations such as radial figures has not been determined, yet.
The aim of this study was to provide the first molecular-cytogenetic characterization of breakpoint distribution and co-localization with FSs, formation of radial figures and involvement of telomeres in formation of chromosomal aberrations in FA-D2 primary lymphocytes originated from patients in different stages of the disease, severe and mild bone marrow failure stage.
Patients and sampling
A total of six patients (five females, one male) previously diagnosed with FA-D2 [3, 10] were included in our study. Mean age of children at the time of study was 8 ± 5 years. Routine control of those children was undertaken at the Mother and Child Health Care Institute of Serbia, Hematooncology department. Disease stage was determined based on complete blood count (CBC) and bone marrow examination using standard criteria. Children were divided into two groups accordingly: severe (group A) or mild (group B) bone marrow failure (BMF). Informed consents from the families were obtained and peripheral blood was collected in Li-heparin vaccutainers for additional testing. The study was approved by The Ethical Committee of Mother and Child Health Care Institute of Serbia.
Whole blood cultures
Aliquots of heparinized whole blood (0.5 ml) were set up in cultures containing PBmax-karyotyping medium (Invitrogen-Gibco, Paisley, UK) and treated with diepoxibuthan (DEB, Sigma Chemicals Co., Germany) (final concentration 0.1 μg/ml) 48 h after culture initiation and further harvested 72 h after initiation. Colchicine (Sigma-Aldrich, Munich, Germany) was added during the last 3 h (final concentration 2.5 μg/ml). Cells were collected by centrifugation and treated with hypotonic solution (0.56 % KCl). Cell suspensions were fixed in methanol/acetic acid (3:1), washed three times with fixative and dropped onto clean slides.
Slides were incubated overnight at room temperature, dehydrated in series of ethanol (70, 95 and 100 %, respectively), 5 min each, and counterstained with 4′,6′-diamidino-2-phenylindole (DAPI)-containing Vectashield solution (Vector Laboratories Ltd, Peterborough, UK). DAPI selectively binds to heterochromatin regions, which produces a banding pattern known as inverse DAPI banding. According to the unique pattern of differentially stained regions each chromosome can be identified. Analysis was performed with a Zeiss-Axioimager A1 microscope and the ISIS imaging software package (MetaSystems, Altlussheim, Germany).
Locus-specific fluorescent in situ hybridization (Locus-specific FISH, BAC-FISH)
BAC-probes used for fluorescence in situ hybridization for the most breakpoint-affected chromosomal regions
Fragile site (FS)
Co-localization of FA breakpoints and FS
Telomere quantitative fluorescent in situ hybridization (Q-FISH)
After metaphase analysis DAPI was removed, slides hybridized with telomere cPNA oligonucleotide probe (TTAGGG) as described by Slijepcevic . Briefly, hybridization was performed with the Cy-3 labeled telomeric PNA probe (CCCTAA) 3′ supplemented with PNA centromeric probe for chromosome 2 (DAKO, Glostrup, Denmark) in final concentration 2 ng/ml. The PNA probe for centromere 2 was added in ready to use telomere probe. After hybridization slides were left in a dark humidified chamber for 2 h. The slides were then washed in 70 % formamide and stained with 4′,6′-diamidino-2-phenylindole (DAPI)-containing mounting medium (Vector Laboratories, UK). Analysis was performed using the ISIS software, MetaSystems (Altlussheim, Germany). Measurements were reported as arbitrary relative telomere length units (RTLU), which are defined as the ratio of signal intensity between telomeres and a centromere chromosome 2 reference signals.
Metaphase spreads were analyzed according to the International System for Human Cytogenetic Nomenclature (ISCN, 2013). For each sample at least 1000 metaphases were analyzed for the breakpoint characterization, whereas at least 500 metaphases were scored for fusions and radial formation with regard to the chromosomes involved in their formation, and involvement of telomeres in arising of those aberrations.
FA breakpoints were expressed as the percentage of total number of breaks per patient. Telomere fusions and radial figures were presented as percentage of total number of metaphases analyzed and were statistically analyzed using nonparametric Mann-Whitney U test in the program SPSS 10 for Windows. Differences at p < 0.05 were considered as significant.
FA breakpoints and assignment to FSs
FA-D2 patients’ karyotypes and molecular cytogenetic findings
Most frequent breakpoints
Fragile site (FS)
Breakpoint frequency (%)
Telomere fusion frequency (%)
Average telomere length (RTLUa ± SD)
Radial figures frequency (%)
distal to FRA1H
14.76 ± 3.23
23.02 ± 12.73
35.72 ± 7.00
20.48 ± 12.24
21.01 ± 10.33
46, XY, der(16)t(1;16)(q42;p11.2)del(1) (q42)/46,XY
23.91 ± 6.92
In contrast, chromosomes in patients of B group tended to break with the higher frequency in the chromosomal regions where the most CFSs are reported (Table 2). Interindividual variability in frequency of the most common breakages was significant in this group, but the breakage pattern in two patients from this group was similar: in patients 4 and 6 the highest frequency of breakage was observed in regions that correspond to FRA3B (11.07 %) and FRA16D (6.87 %), respectively. However, patient 5 had a different breakage pattern compared to other two patients within this group, where low frequencies of the common breakages were found. However, this patient also shows an unbalanced karyotype, mos 46,XX,der(21)t(15;21)(q15;p11.1)/46,XX.
Telomeres and radial figures
The results of our study revealed that chromosomes are not equally prone to breakages in FA-D2 cells exposed to DEB. Matching the data of DAPI banding and BAC-FISH pointed out that regions with increased frequency of breakages co-localize with the CFSs, indicating that frequency of breakpoints as well as their distribution pattern is different among patients and is related to the disease stage.
Assignment of breakpoints to FS in FA-D2 cells is in accordance to previously reported data for FA-A and FA-C cell lines, although the disease stage for the patients in this study was not determined . In FA-D2 patients in the severe stage of the disease (group A) the most frequent breakpoints were present within the CFSs regions (FRA14G, FRA5O, FRA5K and FRA3C, Table 2) except for patient 1 in group A where one of the most frequent breaks was 1q42.2 which is near the FRA1H . However, previously it was not attributed as FS. Their distribution pattern was similar to that found in FA-A and FA-C patients, although different breakpoints were observed in these groups (FRA1A, FRA1D, FRA1E and FRA1J) . The average incidence of chromosomal breaks didn’t increase with the disease progression, but the breakpoint distribution pattern was quite different. Mild stage patients (group B) displayed higher frequencies of breakpoints within the most common FSs, FRA3B and FRA16D except for the patient with unbalanced karyotype (Table 2).
FA pathway plays an essential role in regulation of maintenance and stability of CFSs . Howlett et al. , was the first who establishes that disrupted FA pathway leads to increased chromosomal instability at CFSs underlying that their stability depends on the ATR-FANCD2 signaling pathway, i.e. following DNA replication stress, ATR kinase phosphorylates FANCD2 promoting its monoubiqutination, which activates homologous recombination (HR). Replication stress leads to accumulation of FANCD2 protein in the chromosomal regions prone to breakage, whether they are broken or not ; however altered FANCD2 protein in FA-D2 cells couldn’t maintain stability of existing FSs which in turn activates alternative mechanisms of repair. Apart from HR the CFSs are maintained by non-homologous end-joining (NHEJ) mechanisms, as well as with specialized polymerases that perform by-pass function in DNA synthesis [17, 18].
On the other hand, CFSs are AT-rich sequences prone to form DNA secondary structures which can lead to the replication fork collapse . In addition, replication timing is delayed along CFSs, whereas exposure to DNA polymerase inhibitors intensifies the deceleration of the fork progression, so the CFSs enter G2 phase unreplicated . Both give rise for chromosomal breaks.
Increasing body of evidence now show that large genes constitute the pool of CFSs  indicating relationship between instability and transcription of these genes. Since more than one cell cycle is required for their transcription, transcription and replication machineries collide and CFSs expression may occur . Although different FS distribution pattern with the disease progression remains unclear, it is possible that with the progression of the disease different genes could be expressed as a cellular attempt to compensate progressive genomic instability, leading to different CFSs expression.
Genes located within common fragile sites according to the literature dataa, b, c, d
Molecularly characterized fragile sites
58 600 001
63 800 000
130 800 001
132 900 000
79 200 001
81 600 000
Not-characterized fragile sitesd
147 500 001
150 600 000
106 700 001
111 200 000
214 500 001
220 700 000
183 000 001
184 800 000
44 200 001
50 600 000
69 100 001
74 000 000
153 300 001
156 300 000
104 900 001
107 800 000
70 500 001
75 500 000
73 300 001
78 800 000
66 600 001
70 800 000
61 300 001
63 900 000
Patients in the severe stage of the disease have significantly higher percentage of telomere fusions compared to the patients in the mild stage. Interestingly, there was no significant difference in average telomere length between two groups of patients, but measurement of telomere length at each individual chromosome revealed that the chromosomes with the shortest telomeres were most frequently involved in telomere fusions. Our previous research showed that lymphocyte telomeres in FA-D2 patients are shortened when compared to the age-matched control . Taking into account that FA cells are breakage prone, increased breakages at telomeres and complexity of their function could be the cause of their shortening. Recent review of Holohan et al.  pointed out that impaired telomere maintenance and attrition is a hallmark of FA-D2 group, not FA in general. Our previous study showed that FA-D2 cells displayed heterogeneous telomere length and high frequency of double-strand breaks in telomere regions (telomere dysfunction–induced foci - TIFs) which lead to telomere fragility . Altered FANCD2 protein is not capable to maintain telomeres, leaving the telomeres unprotected and prone to increased fragility and attrition.
FA-D2 patients in the severe stage had significantly more radials than the patients in the mild phase of the disease, particularly radials formed between telomeres of one and interstitial chromosomal regions of other chromosomes, which was rarely observed in group B. This is unreported finding. Since shortened and fragile telomeres act as double strand breaks (DSB) interchromosomal recombination with other impaired chromosomal regions in attempt to repair the damage is not surprising. Additionally, two patients in severe stage developed bone marrow failure several months after cytogenetic examination and became candidates for bone marrow transplantation.
In both groups of patients, radials were composed of non-homologous chromosomes, which is consistent with the previously reported results [27, 28]. Distribution of involved chromosomes was heterogeneous and not specific for the disease stage. However, X chromosome was found in radials in both groups of patients, which is opposite to the report of Newell and colleagues , who found that sex chromosomes are not involved in FA-A and FA-G cells and suggested that alternative mechanisms of ICL repair, which avoid recombination between sex and autosome chromosomes, play the main repair role in FA cells. Involvement of Y chromosome was not observed, but only one male patient in the mild stage and with very low number of radials was part of the study. The presence of sex chromosomes in radial figures in FA patients was not previously reported.
Although radials are described in many genomic instability syndromes, only a few studies clarified the mechanism of their formation. Kuhn and Therman  and Scully et al. , suggested that they arise as an attempt of cellular repair machinery to resolve DSBs, especially when cells with hampered repair machinery are exposed to ICL-s employing homologous recombination [31, 32]. Radials formation in FA cells between non-homologous chromosomes, as well as between autosomes and sex chromosomes indicate that short dispersed chromosomal regions that share homology could act as repair templates in resolving the DNA damage. In FA-D2 cells the X chromosome is not spared from such recombination.
Overall, our results indicate that regions with increased frequency of breakage co-localize with CFSs, whereas their distribution pattern relates to the severity of the disease.
Telomere fusions, as well as interchromosomal recombination and radials formation which involve telomere sequences are the consequence of critically shortened telomeres, increase with disease progression and could be of predictive value.
The authors are thankful to patient, parents and medical staff of the Institute of mother and Child Health Care of Serbia. This work is financially supported by Ministry of Education, Science and Technological Development of the Republic of Serbia ON173046 and DAAD project No 57127754.
This study was funded by Ministry of Education, Science and Technological Development of the Republic of Serbia ON173046 and DAAD project No 57127754.
Availability of data and materials
The data sets supporting the conclusions of this article are included within the article. More details are available upon request.
JF and IJ performed experiments and analytical procedures, and drafted the manuscript. GJ, DV, KM, AW and TL supervised the experiments, edited and approved the manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national). Informed consent was obtained from all patients for being included in the study. Ethics committee of Mother and Child Health Care Institute of Serbia “Dr Vukan Cupic” approved the study and the approval is available upon request.
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