- Case report
- Open Access
TRIP11-PDGFRB fusion in a patient with a therapy-related myeloid neoplasm with t(5;14)(q33;q32) after treatment for acute promyelocytic leukemia
© Kim et al.; licensee BioMed Central. 2014
- Received: 6 October 2014
- Accepted: 15 December 2014
- Published: 23 December 2014
Therapy-related myeloid neoplasm after treatment for acute promyelocytic leukemia (APL) is a relatively infrequent but severe complication. Most therapy-related myeloid neoplasms after treatment for APL are classified as therapy-related myelodysplastic syndrome or therapy-related acute myeloid leukemia. Translocation of 5q31-33, PDGFRB occur rarely in therapy-related myeloid neoplasm and there has been two identified PDGFRB partner genes located at 14q32, TRIP11 and KIAA1509.
The TRIP11-PDGFRB fusion was identified in a patient with therapy-related myeloid neoplasm with t(5;14)(q33;q32) after treatment of APL using conventional cytogenetics, fluorescence in situ hybridization (FISH) and molecular methods. Cytogenetic analysis of the bone marrow aspirate revealed 46, XY, t(5;14)(q33;q32) in all 20 analyzed cells. No other cytogenetic abnormalities were observed. Break-apart FISH analysis demonstrated that rearrangement of PDGFRB at 5q33 was positive in 460 of 500 cells analyzed, while the PML-RARA rearrangement remained undetectable by RT-PCR. Sequencing of RT-PCR products revealed fusion between exon 16 of TRIP11 and exon 11 of PDGFRB. However, the KIAA1509-PDGFRB fusion was not detected by RT-PCR.
We firstly demonstrated that therapy-related myeloid neoplasm with TRIP11-PDGFRB fusion was identified after treatment of APL.
- Therapy-related myeloid neoplasm
- Acute promyelocytic leukemia
Therapy-related myeloid neoplasms (t-MNs) are late complications of cytotoxic therapies used to treat malignant and non-malignant conditions. The incidence of t-MNs is increasing worldwide, due to improved survival rates following treatment of primary malignancies . t-MNs account for 10-20% of all malignant myeloid diagnoses .
The introduction of all trans retinoic acid (ATRA) has been a major breakthrough in the treatment of acute promyelocytic leukemia (APL), characterized by t(15;17)(q22;q12). The combination of ATRA and anthracycline-based chemotherapy results in high rates of complete remission and survival. However, ATRA combined with chemotherapeutic drugs increases the risk of t-MN. Most t-MNs after treatment for APL are classified as therapy related myelodysplastic syndrome (t-MDS) or therapy related acute myeloid leukemia (t-AML). Rearrangement of platelet-derived growth factor receptor beta (PDGFRB) is a distinctive type of myeloid neoplasm which occurs rarely in t-MN. More than 20 different partner genes of PDGFRB have been described . The most common abnormality is the t(5;12)(q31 ~ 33;p12), which forms an ETV6-PDGFRB fusion gene. The TRIP11-PDGFRB fusion gene and the KIAA1509 (HGNC approved gene symbol; CCDC88C)-PDGFRB fusion gene were identified in a patient with myeloproliferative neoplasm (MPN) and t(5;14)(q33;q32) ,. t-MN has an extremely poor clinical outcome. However, t-MNs with PDGFRB rearrangement are very sensitive to imatinib methylate, a tyrosine kinase inhibitor with activity against ABL, c-KIT, and PDGFR.
Here, we report the detection of a TRIP11-PDGFRB fusion in a patient with t-MN with t(5;14)(q33;q32) after treatment of APL.
Conventional cytogenetic analysis and FISH analysis
Chromosomes were analyzed with GTG-banding and the karyotype was described according to the International System for Human Cytogenetics Nomenclature, 2013. FISH analysis was conducted using a dual color PDGFRB breakapart probe (5q33, D5S1907, centromere side, red signal/D5S2014, telomere side, green color). The manufacturer’s recommendations were followed for the hybridization and post-washing procedures (Abbott Molecular/Vysis, Des Plaines, IL, USA). The expected normal signal pattern is two red/green fusion signals. When cells have PDGFRB gene rearrangement, the fusion signal would be split into a red and a green signal.
To determine whether TRIP11 or KIAA1509 were fused to PDGFRB, RNA extraction was performed with the Qiamp RNA Blood Mini Kit (Qiagen, Hilden, Germany). Reverse transcription was performed with random hexamers and MMLV reverse transcriptase (Life Technologies, NY, USA). RT-PCR was performed with Takara LA Taq DNA polymerase (Takara Bio Inc, Shiga, Japan). For the detection of fusion transcript between TRIP11 and PDGFRB, 5′ TRIP11 and 3′ PDGFRB specific primers were used as previously described , using 1407CF (5′-CGCTGCAGCTTTCTGTCTCTCAGGAACAAG-3′) and 2022PR (5′-GTAACGTGGCTTCTTCTGCCA-3′). RT-PCR was performed with ABI 2720 (Life Technologies, Foster City, CA, USA) and cycles were as follows: initial denaturation at 94°C for 1 min; then 35 cycles of 94°C for 30 sec, 60°C for 30 sec and 72°C for 2 min; and a final extension of 5 min at 72°C. Direct sequencing of amplified products was performed with ABI 3130 (Life Technologies). Sequences were analyzed using Sequencher software (version 4.10 Gene Codes Corporation, Ann Arbor, MI, USA). For the detection of a fusion transcript between KIAA1509 and PDGFRB, 5′ KIAA1509 and 3′ PDGFRB specific primers were used as previously described , using KIAA1509-RTF1 (5′-ccgggacacagataagac-3′) and PDGFRB-RTR1 (5′-catgatcttcagctccgaca-3′).
We have identified a TRIP11-PDGFRB fusion in a patient with t(5;14)(q33;q32) after treatment for APL. Cytogenetic analysis of a bone marrow aspirate revealed 46, XY, t(5;14)(q33;q32) in 20 analyzed cells. Break-apart FISH analysis demonstrated that rearrangement of PDGFRB at 5q33 was positive in 460 of 500 cells analyzed. Sequencing of RT-PCR products revealed fusion between exon 16 of TRIP11 (NM_004239.3) and exon 11 of PDGFRB (NM_002609.3), which is the same breakage and reunion point as that reported in the previous study . The KIAA1509-PDGFRB fusion gene was not detected by RT-PCR (Figures 1 and 2). t-MN after treatment for APL is an infrequent but severe complication. The incidence of t-MN was 1% - 6.5% ,. t-MDS is a more common complication than t-AML when considering t-MNs -. The most common cytogenetic abnormalities were partial and complete deletions of chromosomes 5 and 7, and 11q23 rearrangements. Translocation of 5q31-33 and PDGFRB occur rarely in t-MN. The PDGFRB gene at 5q33 is normally expressed in erythroid and myeloid precursors in the bone marrow, and it is a receptor tyrosine kinase which plays an important role in wound healing and other processes in adults ,. PDGFRB is disrupted by other translocations, such as t(5;12), or t(5;14). The most common abnormality is t(5;12)(q31-33;p12), which forms an ETV6-PDGFRB fusion gene. The ETV-PDGFRB fusion gene was identified in patients with chronic myelomonocytic leukemia (CMML) and translocation t(5;12)(q33;p13) by Golub and Gilliland in 1994 . ETV-PDGFRB is a chimeric tyrosine kinase protein which is constitutively active due to enforced homodimerization by self-association domains present on the fusion partner protein (ETV6). This in turn activates downstream signaling pathways, resulting in cell proliferation and survival . Levin et al. cloned KIAA1509 as a PDGFRB fusion partner in imatinib-responsive myeloproliferative disease associated with t(5;14)(q33;q32). Expression of the CEV14-PDGFRB fusion gene has been reported in acute myeloid leukemia with t(7;11) after clonal evolution  in one reported case of t-MN after treatment for AML. Abe et al. hypothesized that the CEV14-PDGFRB fusion causes ectopic constitutive tyrosine kinase activation of PDGFRB, leading to transformation via the ras signal transduction pathway . CEV14 is homologous to TRIP11, which was isolated as one of the proteins that interacts with thyroid hormone receptors in the presence of T3. In our case, response to therapy was monitored by FISH and RT-PCR for the PML-RARA rearrangement. At his last regular follow-up, RT-PCR for PML-PARA was negative, and eosinophilia was mild. Eosinophilia has been reported in recurrent chromosomal aberrations. 5q31-33 is often involved in myeloid disorders with eosinophilia, suggesting involvement of a PDGFRB rearrangement. t(5;14)(q33;q32) was detected with conventional cytogenetics and FISH analysis for PDGFRB was positive. In order to identify partner genes of PDGFRB, such as KIAA1509 or TRIP11, we performed RT-PCR and direct sequencing for both. We have identified a TRIP11-PDGFRB rearrangement. Detection of PDGFRB rearrangement is important because these rearrangements respond well to treatment with imatinib mesylate .
The majority of abnormalities involving PDGFRB are detected by conventional cytogenetics. Performing conventional cytogenetics on a regular basis in all treated APL patients for the early detection of t-MNs implied by chromosomal aberrations is important.
This is the first report of t-MN with a TRIP11-PDGFRB rearrangement after treatment of APL.
Written informed consent was obtained from the patient for publication of this Case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
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