- Case report
- Open Access
A de novo acute myeloid leukemia (AML-M4) case with a complex karyotype and yet unreported breakpoints
© Al-achkar et al.; licensee BioMed Central Ltd. 2013
- Received: 5 March 2013
- Accepted: 18 March 2013
- Published: 5 May 2013
Acute myelogeneous leukemia (AML) is a malignancy of the hematopoietic stem cells, for which cytogenetic analysis is still one of the most important diagnostic and prognostic tools. Still, we are far away from having seen and described all possible genetic changes associated with this kind of acquired disease.
Bone marrow cells of a female patient with clinical diagnoses of AML and immunophenotypically confirmed AML-M4 were studied by GTG-banding. The later was not able to resolve all karyotypic changes and the complex karyotype was characterized in more detail by fluorescence in situ hybridization (FISH) and array-proven multicolor banding (aMCB). To the best of our knowledge, the present case is the only one ever seen with a del(5)(q14q34), a der(17)t(4;17)(p13;p13), a del(2)(p23), a der(4)t(4;7)(p13;q11.23), a der(22)t(11;22)(q23;q11.2) and two complex rearranged chromosomes 11 involving chromosomes 7 and 22 as well as 2.
The yet unreported breakpoints observed in this case seem to be correlated with an adverse prognosis. Overall, molecular cytogenetic studies are suited best for identification and characterization of chromosomal rearrangements in acute leukemia and single case reports as well as large scale studies are necessary to provide further insides in karyotypic changes taking place in human malignancies.
- Acute myeloid leukemia (AML)
- Chromosomal abnormalities
- Fluorescence in situ hybridization (FISH)
- Array-proven multicolor banding (aMCB)
Acute myelogeneous leukemia (AML) is a disease of the myeloid compartment of the hematopoietic system and is characterized by the accumulation of undifferentiated blast cells in the peripheral blood and bone marrow . Cytogenetics is considered the most important independent prognostic parameter in AML [2, 3]. Chromosomal abnormalities also provide useful information for monitoring residual disease . Most of chromosomal abnormalities are detectable by banding cytogenetic analysis, and they occur in 55% of de novo AML in adults [5, 6]. Some chromosomal aberrations in AML are recurrent and closely associated with specific cytomorphological subtypes according to French-American-British (FAB) criteria [7–10]. However, 5-10% of AML patients present with multiple chromosomal rearrangements involving three or more chromosomes. These patients usually have a poor prognosis, and it is likely that some of these rearrangements contribute to their disease progression .
We present a primary AML-M4 case with yet unreported translocation events including seven different chromosomes.
The abnormal cell population (57%) showed the following immunophenotype: CD45+dim(90.4%), HLADr+(86%), CD117+(57%), CD34+(57%), CD18+(60%), CD38+(83%) and expressed CD2 (50%), CD7(24.2%), CD13 (39%), CD33 (20%), CD123 (65%), CD15 (44%) and CD11c (52%) heterogeneously. The abnormal cells negatively reacted with antibodies to CD10, CD64, CD14, CD16, CD5 and CD19. This immunophenotype was consistent with AML-M4 according to FAB classifications.
We described a primary AML-M4 case with cytogenetic rearrangements involving seven different chromosomes. According to the literature, not a single case of AML showed a der(4)t(4;7)(p13;q11.23), a der(11)(11qter->11p11.2::11p11.2->11q23::2p23->2pter), a der(17)t(4;17)(p13;p13), or a der(11)(11pter->11q13::22q11.2->22q13.3::11q13->11q21::7p12->7pter) . However, a t(2;11)(p23;q23) was observed in one case of refractory anemia with excess blasts-1 . To the best of our knowledge, the present case is the only one ever seen case of AML with these cytogenetic aberrations .
The common chromosomal abnormalities in the AML-M4 include monosomy 5 or del(5q), monosomy 7 or del(7q), trisomy 8, t(6;9) (p23;q34), and rearrangements involving the MLL gene mapped at 11q23 [del(11)(q23); t(9;11)(p22;q23), t(11;19)(q23;p13)], and Core Binding Factor B (CBFβ) mapped at 16q22 [del(16)(q22), inv(16)(p13q22), t(16;16)(p13;q22)] . However, in the present case both MLL genes were intact.
In general, a complex karyotype in MDS or AML is associated with a median survival of less than 1 year [11, 14]. Furthermore, the adverse prognostic effect of monosomal karyotype was evident both in the presence and absence of monosomy 5 and/or 7, which suggests that tumor suppressor or other critical genes are not necessarily clustered in specific chromosomes but are instead distributed across several chromosomes .
Monosomy 7 is a valuable prognostic marker in AML, and chromosome 7 defects are prominent cytogenetic lesions in primary myelofibrosis, associated with unfavorable prognosis; they present with high incidences after leukemic transformation . Similarly, deletions on 7p12 of IKZF1 gene (which encodes the transcription factor Ikaros) are associated with a very poor outcome and high relapse rate in B-cell acute lymphocytic leukemia . Monosomy 7 is known as a recurrent cytogenetic aberration in approximately 10% of adult and 5% of childhood AML cases . Jäger et al.  found two of seven myeloproliferative neoplasms patients with loss of IKZF1 had monosomy 7. This result suggests that IKZF1 may represent an important tumor-suppressor gene affected by monosomy 7 .
The International Prognostic Scoring System (IPSS) classifies cytogenetic and molecular genetic data in AML with clinical data into four risk groups: favorable, intermediate-I, intermediate-II and adverse . The adverse prognostic groups included inv(3)(q21q26.2) or t(3;3)(q21;q26.2); RPN1-EVI1; t(6;9)(p23;q34); DEK-NUP214; t(v;11)(v;q23); MLL rearranged; -5 or del(5q); -7; abnl(17p); complex karyotype .
Complex karyotypes, which occur in 10-12% of AML patients, have consistently been associated with a very poor outcome . A complex karyotype has been defined as the presence of 3 or more (in some studies ≥ 5) chromosome abnormalities. For AML it turned out that the presence of t(8;21), inv(16) or t(16;16), and t(15;17) ameliorates the adverse effect of increase karyotypic complexity . As indicated in the new WHO classification, cases with other recurring genetic abnormalities, such as t(9;11) or t(v;11), inv(3) or t(3;3), and t(6;9) should also be excluded from complex rearranged karyotype patient group , because these groups constitute separate entities. One striking observation is the increasing incidence of adverse versus favorable cytogenetic abnormalities with increasing age. This, at least in part, contributes to the poorer outcome of AML in older adults .
In conclusion, we reported a de novo case of AML-M4 with yet unreported translocation events involving seven different chromosomes. Taken together all findings an adverse prognosis for this specific AML-case must be considered.
A 65-year-old woman was diagnosed as suffering from AML in September 2011. Anemia, thrombocytopenia, fever, fatigue and weight loss were the indicative symptoms. Her hematologic parameters were: white blood cells (WBC) of 34.2×109/l with 25.5% neutrophils, 36.2% lymphocytes, and 38.3% immature cells, red blood cell (RBC) count was 1.86×106/mm3, hemoglobin level was 6.7 g/dl and the platelet count was 19×109/l. No treatment had been administered prior to the tests mentioned below. All human studies have been approved by the ethics committee of the Atomic Energy Commission, Damascus, Syria and have therefore been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments. The patient gave his informed consent prior to its inclusion in this study. Later the patient was lost during follow-up.
Chromosome analysis using GTG-banding was performed according to standard procedures . A minimum of 20 metaphase cells derived from unstimulated bone marrow culture were analyzed. Karyotypes were described according to the International System for Human Cytogenetic Nomenclature .
Fluorescence in situ hybridization (FISH) using LSI BCR/ABL dual color dual fusion translocation probe (Abbott Molecular/Vysis, Des Plaines, IL, USA), MLL break-apart probe (Q-Biogene, USA) mixed with LSI BCR/ABL dual color dual fusion translocation probe chromosome enumeration probe (CEP) for chromosomes 9 and 11 (Abbott Molecular /Vysis) and 17p13 (p53), dual color probe (Q-Biogene, USA) were applied according to manufacturer’s instructions. Whole chromosome painting (WCP) probes for chromosomes 2, 4, 5, 7, 11, 17 and 22 were also applied (MetaSystems, Altlussheim, Germany) . FISH using the corresponding chromosome specific array-proven multicolor banding (aMCB) probe sets based on microdissection derived region-specific libraries was performed as previously reported . A minimum of 20 metaphase spreads were analyzed, using a fluorescence microscope (AxioImager.Z1 mot, Carl Zeiss Ltd., Hertfordshir, UK) equipped with appropriate filter sets to discriminate between a maximum of five fluorochromes plus the counterstain DAPI (4′,6- diamino-2-phenylindole). Image capture and processing were performed using an ISIS imaging system (MetaSystems).
Flow cytometric immunophenotype
Flow cytometric analysis was performed using a general panel of fluorescent antibodies against the following antigens typical for different cell lineages and cell types: CD1a, CD2, CD3, CD4, CD5, CD8, CD10, CD11b, CD11c, CD13, CD14, CD15, CD16, CD19, CD20, CD22, CD23, CD32, CD33, CD34, CD38, CD41a, CD45, CD56, CD57, CD64, CD103, CD117, CD123, CD138, CD209, CD235a and CD243; In addition to antibodies to Kappa and Lambda light Chains, IgD, sIgM, and HLADr. All antibodies purchased from BD Biosciences. Samples analyzed on a BD FACSCalibur™ flow cytometer. Autofluorescence, viability, and isotype controls were included. Flow cytometric data acquisition and analysis were conducted by BD Cellquest™ Pro software.
We thank Prof. I. Othman, the Director General of Atomic Energy Commission of SYRIA (AECS) and Dr. N. Mirali, Head of Molecular Biology and Biotechnology Department for their support. This work was supported by the AECS, in parts by the DAAD, Stefan-Morsch-Stiftung and the Monika-Kutzner-Stiftung.
- Rubnitz JE: Childhood acute myeloid leukemia. Curr Treat Options Oncol 2008, 9: 95–105. 10.1007/s11864-008-0059-zView ArticlePubMedGoogle Scholar
- Byrd JC, Mrozek K, Dodge RK, Carroll AJ, Edwards CG, Arthur DC, Pettenati MJ, Patil SR, Rao KW, Watson MS, Koduru PR, Moore JO, Stone RM, Mayer RJ, Feldman EJ, Davey FR, Schiffer CA, Larson RA, Bloomfield CD, Cancer and Leukemia Group B (CALGB8461): Pretreatment cytogenetic abnormalities are predictive of induction success, cumulative incidence of relapse, and overall survival in adult patients with de novo acute myeloid leukemia: results from Cancer and Leukemia Group B (CALGB 8461). Blood 2002, 100: 4325–4336. 10.1182/blood-2002-03-0772View ArticlePubMedGoogle Scholar
- Grimwade D, Walker H, Oliver F, Wheatley K, Harrison C, Harrison G, Rees J, Hann I, Stevens R, Burnett A, Goldstone A: The importance of diagnostic cytogenetics on outcome in AML: analysis of 1,612 patients entered into the MRC AML 10 trial. The medical research council adult and children’s leukaemia working parties. Blood 1998, 92: 2322–2333.PubMedGoogle Scholar
- Grimwade D, Lo Coco F: Acute promyelocytic leukemia: a model for the role of molecular diagnosis and residual disease monitoring in directing treatment approach in acute myeloid leukemia. Leukemia 2002, 16: 1959–1973. 10.1038/sj.leu.2402721View ArticlePubMedGoogle Scholar
- Mrozek K, Heinonen K, Bloomfield CD: Prognostic value of cytogenetic findings in adults with acute myeloid leukemia. Int J Hematol 2000, 72: 261–271.PubMedGoogle Scholar
- Mrozek K, Heinonen K, Bloomfield CD: Clinical importance of cytogenetics in acute myeloid leukaemia. Best Pract Res Clin Haematol 2001, 14: 19–47. 10.1053/beha.2000.0114View ArticlePubMedGoogle Scholar
- Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DA, Gralnick HR, Sultan C: Proposals for the classification of the acute leukaemias. French-American-British (FAB) co-operative group. Br J Haematol 1976, 33: 451–458. 10.1111/j.1365-2141.1976.tb03563.xView ArticlePubMedGoogle Scholar
- Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DA, Gralnick HR, Sultan C: Proposed revised criteria for the classification of acute myeloid leukemia. A report of the French-American-British Cooperative Group. Ann Intern Med 1985, 103: 620–625. 10.7326/0003-4819-103-4-620View ArticlePubMedGoogle Scholar
- Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DA, Gralnick HR, Sultan C: Criteria for the diagnosis of acute leukemia of megakaryocyte lineage (M7). A report of the French-American-British Cooperative Group. Ann Intern Med 1985, 103: 460–462. 10.7326/0003-4819-103-3-460View ArticlePubMedGoogle Scholar
- Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DA, Gralnick HR, Sultan C: Proposal for the recognition of minimally differentiated acute myeloid leukemia (AML-M0). Br J Haematol 1991, 78: 325–329. 10.1111/j.1365-2141.1991.tb04444.xView ArticlePubMedGoogle Scholar
- Haase D, Germing U, Schanz J, Pfeilstöcker M, Nösslinger T, Hildebrandt B, Kundgen A, Lübbert M, Kunzmann R, Giagounidis AA, Aul C, Trümper L, Krieger O, Stauder R, Müller TH, Wimazal F, Valent P, Fonatsch C, Steidl C: New insights into the prognostic impact of the karyotype in MDS and correlation with subtypes: evidence from a core dataset of 2124 patients. Blood 2007, 110: 4385–4395. 10.1182/blood-2007-03-082404View ArticlePubMedGoogle Scholar
- Mitelman F, Johansson B, Mertens F (Eds): In Mitelman database of chromosome aberrations and gene fusions in cancer. 2012. Available at: [accessed 26.11.2012] http://cgap.nci.nih.gov/Chromosomes/MitelmanGoogle Scholar
- Chen Z, Sandberg AA: Molecular cytogenetic aspects of hematological malignacies: clinical implications. Am J Med Genet 2002, 115: 130–141. 10.1002/ajmg.10689View ArticlePubMedGoogle Scholar
- Pozdnyakova O, Miron PM, Tang G, Walter O, Raza A, Woda B, Wang SA: Cytogenetic abnormalities in a series of 1,029 patients with primary myelodysplastic syndromes: a report from the US with a focus on some undefined single chromosomal abnormalities. Cancer 2008, 113: 3331–3340. 10.1002/cncr.23977View ArticlePubMedGoogle Scholar
- Patnaik MM, Hanson CA, Hodnefield JM, Knudson R, Van Dyke DL, Tefferi A: Monosomal karyotype in myelodysplastic syndromes, with or without monosomy 7 or 5, is prognostically worse than an otherwise complex karyotype. Leukemia 2011, 25: 266–270. 10.1038/leu.2010.258View ArticlePubMedGoogle Scholar
- Mesa R, Li C, Ketterling R, Schroeder G, Knudson R, Tefferi A: Leukemic transformation in myelofibrosis with myeloid metaplasia: a single-institution experience with 91 cases. Blood 2005, 105: 973–977.View ArticlePubMedGoogle Scholar
- Mullighan CG, Miller CB, Radtke I, Phillips LA, Dalton J, Ma J, White D, Hughes TP, Le Beau MM, Pui CH, Relling MV, Shurtleff SA, Downing JR: BCR-ABL1 lymphoblastic leukaemia is characterized by the deletion of Ikaros. Nature 2008, 453: 110–114. 10.1038/nature06866View ArticlePubMedGoogle Scholar
- Luna-Fineman S, Shannon KM, Lange BJ: Childhood monosomy 7: epidemiology, biology, and mechanistic implications. Blood 1995, 85: 1985–1999.PubMedGoogle Scholar
- Jäger R, Gisslinger H, Passamonti F, Rumi E, Berg T, Gisslinger B, Pietra D, Harutyunyan A, Klampfl T, Olcaydu D, Cazzola M, Kralovics R: Deletions of the transcription factor Ikaros in myeloproliferative neoplasms. Leukemia 2010, 24: 1290–1298. 10.1038/leu.2010.99View ArticlePubMedGoogle Scholar
- Döhner H, Estey EH, Amadori S, Appelbaum FR, Büchner T, Burnett AK, Dombret H, Fenaux P, Grimwade D, Larson RA, Lo-Coco F, Naoe T, Niederwieser D, Ossenkoppele GJ, Sanz MA, Sierra J, Tallman MS, Löwenberg B, Bloomfield CD, European LeukemiaNet: Diagnosis and management of acute myeloid leukemia in adults: recommendations from an international expert panel, on behalf of the European LeukemiaNet. Blood 2010, 115: 453–474. 10.1182/blood-2009-07-235358View ArticlePubMedGoogle Scholar
- Mrozek K: Cytogenetic, molecular genetic, and clinical characteristics of acute myeloid leukemia with a complex karyotype. Semin Oncol 2008, 358: 365–377.View ArticleGoogle Scholar
- Vardiman JW, Thiele J, Arber DA, Brunning RD, Borowitz MJ, Porwit A, Harris NL, Le Beau MM, Hellström-Lindberg E, Tefferi A, Bloomfield CD: The 2008 revision of the WHO classification of myeloid neoplasms and acute leukemia: rationale and important changes. Blood 2009, 114: 937–951. 10.1182/blood-2009-03-209262View ArticlePubMedGoogle Scholar
- Appelbaum FR, Gundacker H, Head DR, Slovak ML, Willman CL, Godwin JE, Anderson JE, Petersdorf SH: Age and acute myeloid leukemia. Blood 2006, 107: 3481–3485. 10.1182/blood-2005-09-3724PubMed CentralView ArticlePubMedGoogle Scholar
- Al-Achkar W, Wafa A, Nweder MS: A complex translocation t(5;9;22) in Philadelphia cells involving the short arm of chromosome 5 in a case of chronic myelogenous leukemia. J Exp Clin Cancer Res 2007, 26: 411–415.PubMedGoogle Scholar
- Shaffer LG, Slovak ML, Cambell LJ: ISCN. International system for human cytogenetic nomenclature. S Karger AG: Basel; 2009.Google Scholar
- Weise A, Mrasek K, Fickelscher I, Claussen U, Cheung SW, Cai WW, Liehr T, Kosyakova N: Molecular definition of high-resolution multicolor banding probes: first within the human DNA sequence anchored FISH banding probe set. J Histochem Cytochem 2008, 56: 487–493. 10.1369/jhc.2008.950550PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.