Chromosomal instability (CIN) in HAP1 cell lines revealed by multiplex fluorescence in situ hybridisation (M-FISH)

Table 4 CIN associated with chromosome 13 in near-haploid HAP1 cell lines

HAP1 Cell line ID	Non-reciprocal translocation involving chromosome 13 [1n & 2n]		Robertsonian translocation involving chromosome 13[1n & 2n]		Deletion involving chromosome 13[1n & 2n]		N-CIN involving chromosome 13[1n & 2n]
	[1n]	[2n]	[1n]	[2n]	[1n]	[2n]	[1n]	[2n]
HAP1 Pool Lig4- Cas9-								− 13[2n]
B3 HAP1 P17				rob(13;13)[2n]
	der(13)t(9;13)[1n]
HAP1 A1					+ del(13)[1n]
HAP1 E5	der[13;(22)t(9;22)], + der[13;(22)t(9;22)] × 3[1n]
					+del(13)[1n]
							+13[1n]
				rob(13;13)[2n]
HAP1 F3				rob(13;13)[2n]
								− 13[2n]
								+13 × 3[3n]
HAP1 G2	der(13)t[der(22)][1n]
HAP1-HO-C	der(13)t(3;13)[1n]
	der(13)t(13;20)[1n]
	der(13)t(5;13)[1n]
HAP1 GR 5.10	der(13)t(8;13)[1n]
	der(13)t(9;13)[1n][4]*
	der(13)t[dup(9);13][1n]
			rob(13;13)[1n]
			rob(13;15)[1n]
			rob(13;21)[1n]
	der(13)t(X;13)[1n]
4C1 R1				rob(13;13)[2n]
				rob(13;13)[2n]
4C1 R2		der(13)t(13;15;22) × 2[2n]
				rob(13;13)[2n]
19C1 R1				rob(13;15)[2n]
				rob(13;14) × 2[2n]
						del(13)[2n]
HAP1 Pool Cas9+Blast	der(13)t(9;13)
				rob(13.13)[2n]
								− 13[2n]

Novel N- and S-CIN associated with chromosome 13 in near-haploid HAP1 cell lines; * indicates number of metaphases

ISSN: 1755-8166