Fig. 2

Molecular analysis of leukemia markers. a Confirmation of the AML-ETO fusion was done by Taq Man real-time PCR (Amplification plot). (i) The presented case carried AML-ETO fusion translocation t(8:21), thereby confirming the AML/ETO fusion positivity. (ii) The graph for positive and negative controls are shown along with endogenous ABL gene amplification. The x-axis shows the number of PCR cycles and the y-axis shows the normalized fluorescence intensity. The threshold cycle values are calculated automatically by determining the point at which the fluorescence exceeds a fixed threshold line. b Fragment analysis method based on capillary electrophoresis for FLT3 INDEL detection. (i) Blue peaks correspond to the FLT3 fragment detection and red peaks correspond to the marker size standards. A wild type patient showing the amplicon size expected. (ii) A mutated patient showed an insertion with an additional peak to the wild type