Fig. 5From: The rare mutation in the endosome-associated recycling protein gene VPS50 is associated with human neural tube defectsa Distribution of the four categories in each of the experimental groups. We clustered these injected embryos into four categories according to the severity of their morphology. Grade 1: WT like; Grade 2: mild, curving axis compared with WT embryos; Grade 3: moderate, up to 1/3 shortened axis; Grade 4: severe, the body axis just extended out of the range of the yolk ball. b Injection of vps50-MOs yielded high proportion abnormal phenotypes. Wild-type VPS50 exhibited higher efficiency saving ability than the p. Gly169Val mutation. Dosage-dependent rescue of vps50 knock-down zebrafish embryo with Human VPS50 mRNA. 6 ng vps50-MOs were coinjected with 0, 200, and 400 pg of VPS50 mRNA. The number above each bar was the total number of embryos counted. The P value was calculated by chi-squared analysisBack to article page