Fig. 2

Electrophoretic control of the fragments of Y14–Myc construction. a, RNA electrophoresis. I — RNA electrophoresis under denaturing conditions; line 1, total RNA from rat liver (for control); line 2, total T. castaneum RNA from pupae. II — RNA transcribed in vitro and analyzed with microfluidics-based Agilent 2100 Bioanalyzer; line 1, a ladder (Agilent 2100 Bioanalyzer built-in function); lines 2 and 3, 5'-capped T. castaneum Y14-Myc RNA transcribed in vitro, 200 ng and 100 ng, respectively; line 4, mouse GABDH mRNA (for control, 200 ng). b, DNA electrophoresis. I — DNA electrophoresis of the reverse transcription PCR products; line 1, DNA ladder (100 bp); line 2, negative control; line 3, PCR with cDNA after RT-PCR with total T. castaneum RNA (506 bp), primers ## 1, 2 (see Table 1). II — DNA electrophoresis of PCR products amplified with Pfu polymerase on the PTZ19R + Y14-Myc vector for in vitro transcription (639 bp); line 1, DNA ladder (100 bp); line 2, primers ## 5, 4 (Table 1); line 3, primers ## 3, 6 (Table 1); line 4, primers ## 5, 6 (Table 1)