Fig. 4

a SP 20 μM, after 48 h of treatment with SP600125, the caspase-3 activity was assayed using a caspase assay kit, following the manufacturer’s protocol. b Quantification of Multinucleated cells observed because of the SP600125 incubation (20 μM). Time dependent experience. The average value ± SD from three independent experiments is also shown. Asterisks indicate significant differences (* p < 0.05) calculated by the Duncan’s t-test. c Equal amounts of cell lysate (60 μg) were resolved using SDS-PAGE, transferred to nitrocellulose, and probed with specific antibodies (anti-PARP and anti-Bcl-2). Actin was used as an internal loading control