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Fig. 3 | Molecular Cytogenetics

Fig. 3

From: Application of a modified drop method for high-resolution pachytene chromosome spreads in two Phalaenopsis species

Fig. 3

FISH mapping of 45S rDNA (arrowheads) on chromosomes at pachytene stage (a) and metaphase (b) in P. aphrodite subsp. formosana. Telomere DNA in green (b) was also mapped on metaphase chromosomes. c The pachytene and metaphase chromosomes with 45S rDNA signals were straightened using ImageJ software. The asterisk and pound sign indicate the corresponding chromosomes in (b). Chromosomes were stained with DAPI and images were converted to black and white. Scale bar = 10 μm

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