Fig. 3
From: The major horse satellite DNA family is associated with centromere competence
a: Immuno-FISH on mechanically stretched chromosomes. 37cen is red labelled while CENP-A, detected by a CENP-A enriched CREST serum is green labelled. A total number of 99 stretched chromosomes was analysed. A sample of representative images is reported in the figure. b: Immuno-FISH on extended chromatin fibres. The 37cen satellite DNA is labelled in red. CENP-A, identified with a CENP-A enriched CREST serum is green labelled. In each panel, under the microscope image of the fibre, the CENP-A binding pattern observed is sketched. Images on the right show line graphs quantifying the fluorescence staining along the length of each fibre. I: CENP-A covers the whole length of the 37cen positive region. II: CENP-A binding regions are arranged in blocks of variable length intermingled in the 37cen positive stretch. III: A chromatin fibre with no CENP-A binding is reported