Molecular cytogenetic characterization of Dasypyrum breviaristatum chromosomes in wheat background revealing the genomic divergence between Dasypyrum species

Li, Guangrong; Gao, Dan; Zhang, Hongjun; Li, Jianbo; Wang, Hongjin; La, Shixiao; Ma, jiwei; Yang, Zujun

doi:10.1186/s13039-016-0217-0

Molecular Cytogenetics

Table 1 The primers used to localize the Vb7 specific amplification in D2139

From: Molecular cytogenetic characterization of Dasypyrum breviaristatum chromosomes in wheat background revealing the genomic divergence between Dasypyrum species

No.	Primers name	Primer sequences (5’–3’)	Wheat bin map	Restriction enzyme	Length of Vb7 bands
1	TNAC1776	F: ATCATCCTGCTGCTACTGTGC	7AS2-0.73–0.83	–
		R: CCTTCTCAGCTTAGCGATGTG	4AL2-0.75–0.80
		R: CCTTCTCAGCTTAGCGATGTG	7DS4-0.73–1.00
2	TNAC1782	F: TCACTGAACAGCCTAGACATGG	7AS2-0.73–0.83	HaeIII	690 bp
		R: ATTCGCAGACCGCATCTATC	7BS2-0.27–1.00
		R: ATTCGCAGACCGCATCTATC	7DS4-0.73–1.00
3	TNAC1803	F: TGCGACCAGTCTCTTTGAAAT	C-7AL1-0.39	HaeIII	800 bp*
		R: GTCGGAGCCTGGATCTCTAGT	7BL2-0.38–0.63
		R: GTCGGAGCCTGGATCTCTAGT	7DL5-0.30–0.61
4	TNAC1806	F: ATTCCTCGTGAATTGCTGGAT	7AS8-0.45–0.59	TaqI	350 bp*
		R: TCTGCAGTTAGGGACTTGAAA	7BS2-0.27–1.00
		R: TCTGCAGTTAGGGACTTGAAA	7DS2-0.61–0.73
5	TNAC1811	F: CTGCTCAACGAGTTCATCGAC	7AL1-0.39–0.63	TaqI	740 bp
		R: TTGGAGTGGACGTTGCATT	7BL2-0.38–0.63
		R: TTGGAGTGGACGTTGCATT	7DL5-0.30–0.61
6	TNAC1812	F: ACTTCGCTTGGTCTCCTCAAT	7AL5-0.63–0.71	TaqI	860 bp
		R: GAGAAGTGTGCCAATTCCAAA	7BL7-0.63–0.78
		R: GAGAAGTGTGCCAATTCCAAA	7DL5-0.30–0.61
7	TNAC1815	F: AGCAGACATCAGCAAGTTTGAG	7AL1-0.39–0.63	TaqI	600 bp*
		R: ACTGACAAGCCCATGATTGAC	7BL2-0.38–0.63
		R: ACTGACAAGCCCATGATTGAC	7DL5-0.30–0.61
8	TNAC1822	F: CCCTCCGTCCGTGCAAAT	7AL5-0.63–0.71	TaqI	730 bp*
		R: GGCTGATGATGGAGACGTG	7BL2-0.38–0.63
		R: GGCTGATGATGGAGACGTG	7DL2-0.61–0.82
9	TNAC1867	F: GCCTTTCCTTTGGTAGTCTGG	C-7AL1-0.39	–	840 bp
		R: CGATCCAAATGATCCTGAAGA	7BL2-0.38–0.63
		R: CGATCCAAATGATCCTGAAGA	7DL1-0.14–0.30
10	TNAC1903	F: TCGCTTCTTCTGCTTGTTCTT	C-7AL1–0.39	TaqI	920 bp*
		R: CTGCTACTAGGCCACCCAAA	C-7BL2–0.38
		R: CTGCTACTAGGCCACCCAAA	7DL1-0.14–0.30
11	TNAC1926	F: CGTCAGCTACAGCGACATCTA	C-7AS8–0.45	TaqI	700 bp*
		R: AACTTGAGCAGCGTGGTGTT	7BS2-0.27–1.00
		R: AACTTGAGCAGCGTGGTGTT	7DS3-0.15–0.36
12	TNAC1943	F: GCTGCTATGGTCCACGAATTA	7AS5-0.59–0.73	HaeIII	600 bp*
		R: AGAGTATCGTATCCGGGCAAT	7BS2-0.27–1.00
		R: AGAGTATCGTATCCGGGCAAT	7DS4-0.73–1.00
13	TNAC1957	F: TCAACATTTGCAGGATTGTCA	7AL21-0.74–0.86	–	730 bp
		R: TTTCACAGGAACCTCTGCATC	7BL10-0.78–0.84
		R: TTTCACAGGAACCTCTGCATC	7DL2-0.61–0.82

The primers and the location in wheat bin map were referred to Ishikawa et al. [18]. The star indicated the Vb7 specific bands were polymorphic to 7V band

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ISSN: 1755-8166

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