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Table 1 Analysis of semen samples using FISH with two DNA-probes from chromosome 21, Vysis 21q22 (red) and Cytocell 21qtel (green) together with a control chromosome 18 probe, Abbot (blue), to allow differentiation between disomy and diploidy (or disomy 21 plus disomy 18)

From: Disomy 21 in spermatozoa and the paternal origin of trisomy 21 Down syndrome

No of signals from the two chr 21 DNA probes 1green/1red 2green/2red 2green/1red1 1green/2red1 1green/0red1 0green/1red1 Total no of cells
(normal) (disomy)
Sample and age of the men HP1 1988 (99.4 %) 5 (0.25 %) 3 (0.15 %) 4 (0.2 %) 0 (0.00 %) 0 (0.00 %) 2000
32 y
HP2 1994 (99.7 %) 3 (0.15 %) 0 (0.00 %) 3 (0.15 %) 0 (0.00 %) 0 (0.00 %) 2000
37 y
HP3 1992 (99.6 %) 2 (0.10 %) 2 (0.10 %) 4 (0.2 %) 0 (0.00 %) 0 (0.00 %) 2000
39 y
HP4 1991 (99.55 %) 1 (0.05 %) 4 (0.2 %) 4 (0.2 %) 0 (0.00 %) 0 (0.00 %) 2000
33 y
HP5 1978 (98.9 %) 0 (0.00 %) 3 (0.15 %) 14 (0.7 %) 5 (0.25 %) 0 (0.00 %) 2000
45 y
3486 1991 (99.55 %) 3 (0.15 %) 4 (0.2 %) 2 (0.10 %) 0 (0.00 %) 0 (0.00 %) 2000
28 y
3499 1993 (99.65 %) 4 (0.2 %) 2 (0.10 %) 1 (0.05 %) 0 (0.00 %) 0 (0.00 %) 2000
53 y
3500 1992 (99.6 %) 2 (0.10 %) 2 (0.10 %) 3 (0.15 %) 0 (0.00 %) 1 (0.05 %) 2000
30 y
3501 1986 (99.3 %) 2 (0.10 %) 1 (0.05 %) 9 (0.45 %) 0 (0.00 %) 2 (0.10 %) 2000
42 y
3502 1992 (99.6 %) 5 (0.25 %) 1 (0.05 %) 1 (0.05 %) 1 (0.05 %) 0 (0.00 %) 2000
40 y
3505 1995 (99.75 %) 1 (0.05 %) 0 (0.00 %) 3 (0.15 %) 1 (0.05 %) 0 (0.00 %) 2000
35
Mean 1990 (99.51 %) 2.5 (0.13 %) 2.0 (0.10 %) 4.4 (0.22 %) 0.6 (0.03 %) 0.3 (0.01 %)  
SD 4.5 (0.23 %) 1.6 (0.08 %) 1.3 (0.07 %) 3.7 (0.18 %) 1.4 (0.07 %) 0.6 (0.03 %)
  1. 1Those spermatozoa showing either 2 green/1 red, 1 green/2 red, 1 green/0 red or 0 green/1 red signals were interpreted as false positives or false negatives, respectively, and were therefore excluded in the results of true disomy 21
  2. Standard deviation (SD)