Table 1 Analysis of semen samples using FISH with two DNA-probes from chromosome 21, Vysis 21q22 (red) and Cytocell 21qtel (green) together with a control chromosome 18 probe, Abbot (blue), to allow differentiation between disomy and diploidy (or disomy 21 plus disomy 18)
From: Disomy 21 in spermatozoa and the paternal origin of trisomy 21 Down syndrome
No of signals from the two chr 21 DNA probes | 1green/1red | 2green/2red | 2green/1red1 | 1green/2red1 | 1green/0red1 | 0green/1red1 | Total no of cells | |
|---|---|---|---|---|---|---|---|---|
(normal) | (disomy) | |||||||
Sample and age of the men | HP1 | 1988 (99.4 %) | 5 (0.25 %) | 3 (0.15 %) | 4 (0.2 %) | 0 (0.00 %) | 0 (0.00 %) | 2000 |
32 y | ||||||||
HP2 | 1994 (99.7 %) | 3 (0.15 %) | 0 (0.00 %) | 3 (0.15 %) | 0 (0.00 %) | 0 (0.00 %) | 2000 | |
37 y | ||||||||
HP3 | 1992 (99.6 %) | 2 (0.10 %) | 2 (0.10 %) | 4 (0.2 %) | 0 (0.00 %) | 0 (0.00 %) | 2000 | |
39 y | ||||||||
HP4 | 1991 (99.55 %) | 1 (0.05 %) | 4 (0.2 %) | 4 (0.2 %) | 0 (0.00 %) | 0 (0.00 %) | 2000 | |
33 y | ||||||||
HP5 | 1978 (98.9 %) | 0 (0.00 %) | 3 (0.15 %) | 14 (0.7 %) | 5 (0.25 %) | 0 (0.00 %) | 2000 | |
45 y | ||||||||
3486 | 1991 (99.55 %) | 3 (0.15 %) | 4 (0.2 %) | 2 (0.10 %) | 0 (0.00 %) | 0 (0.00 %) | 2000 | |
28 y | ||||||||
3499 | 1993 (99.65 %) | 4 (0.2 %) | 2 (0.10 %) | 1 (0.05 %) | 0 (0.00 %) | 0 (0.00 %) | 2000 | |
53 y | ||||||||
3500 | 1992 (99.6 %) | 2 (0.10 %) | 2 (0.10 %) | 3 (0.15 %) | 0 (0.00 %) | 1 (0.05 %) | 2000 | |
30 y | ||||||||
3501 | 1986 (99.3 %) | 2 (0.10 %) | 1 (0.05 %) | 9 (0.45 %) | 0 (0.00 %) | 2 (0.10 %) | 2000 | |
42 y | ||||||||
3502 | 1992 (99.6 %) | 5 (0.25 %) | 1 (0.05 %) | 1 (0.05 %) | 1 (0.05 %) | 0 (0.00 %) | 2000 | |
40 y | ||||||||
3505 | 1995 (99.75 %) | 1 (0.05 %) | 0 (0.00 %) | 3 (0.15 %) | 1 (0.05 %) | 0 (0.00 %) | 2000 | |
35 | ||||||||
Mean | 1990 (99.51 %) | 2.5 (0.13 %) | 2.0 (0.10 %) | 4.4 (0.22 %) | 0.6 (0.03 %) | 0.3 (0.01 %) | ||
SD | 4.5 (0.23 %) | 1.6 (0.08 %) | 1.3 (0.07 %) | 3.7 (0.18 %) | 1.4 (0.07 %) | 0.6 (0.03 %) | ||