Fig. 2
From: Chromosome territory repositioning induced by PHA-activation of lymphocytes: A 2D and 3D appraisal
Examples of 3D models demonstrating the various methods of analysis. A representative image of 3D models and measurements performed using Imaris (V.7.6.3) is provided for CT 17 (red) and CT 22 (green) in a resting (-PHA) and activated (+PHA) lymphocyte nucleus with a schematic representation shown below. Panels a-e, each show the same same nucleus and provides an example of each measurement performed. Panel a: diameter of the nucleus (-PHA 10.4 μm; +PHA 17.1 μm). Panel b: geometrical center of each CT, which is utilized by the distance transformation (DT) as the point to measure to the nearest nuclear edge in any direction (shown for CT 17 -PHA; and CT 22 + PHA). DT measurements for each nucleus are: CT 17: 1.84 μm, 3.29 μm and CT 22 3.43 μm, 3.97 μm (-PHA), CT 17 2.68 μm, 3.23 μm and CT 22 1.5 μm 1.93 μm (+PHA). Panel c: homologous CT measurements between the center of each CT (CT 17 5.34 μm, CT 22 2.44 μm -PHA; and CT 17 7.02 μm, CT 22 4.95 μm + PHA). Panel d: heterologous CT measurements between the center of the closet heterologous CT pair (CT 17–22 2.41 μm -PHA; and CT 17–22 4.58 μm + PHA) and the furthest heterologous CT pair (CT 17–22 2.41 μm -PHA; and CT 17–22 4.58 μm + PHA). Panel e: the same cell that has been rotated toward the left with 50 % of the DAPI plane removed “clipped” to better demonstrate the 3D aspect of the CTs within a 3D nucleus