Fig. 1

Representation samples of copy number changes observed in samples biopsied from blastocysts. Left panel: PGS results from array comparative genomic hybridization (aCGH) analysis; right panel: PGS results from next generation sequencing (NGS) analysis. X-axis indicate chromosome numbers (1-22, X and Y) and y-axis indicate chromosome copy number assignments (0, 1, 2, 3, or 4). The gains (copy number state >2) and losses (copy number state <2) of chromosomes in the right panel obtained with NGS exactly match those in the left panel obtained with aCGH. (I) Top left and right charts show a monosomy X from sample #3 in Table 2. (II) Middle left and right charts show monosomy 4, 5, 18 and 19 from sample #9 in Table 2. (III) Bottom left and right charts show trisomy 11, 14, 22 and monosomy19 from sample #35 in Table 2. Arrows indicate the locations of abnormal chromosomes