Genomic instability of human embryonic stem cell lines using different passaging culture methods

Table 1 Recurrent genomic variations acquired during hESC H1 and H9 culture

Chromosome region	Size (kb min-max)	Common genes	H1 M early	H1 E early	H1 E late	H9 M early	H9 E early	H9 E late
1q21.3	12.42-25.84	LCE3C⁴	L	-	L	G	-	G
4p16.3	27.74-253.75	FAM53A	-	-	-	-	G	L
6p21.32	37.12-81.43	HLA-DRB5⁴	L	L	L	G	G	G
11q11	54.62-82.97	OR4C11, OR4P4, OR4S2	L	L	L	L	-	L
14q23.2	295.35	KCNH5, RHOJ³, GPHB5	-	-	-	G	-	G
14q23.3	398.69	FUT8³	-	-	-	G	-	G
15q11.2	1270.33-1779-68	LOC283755, A26B1, OR4M2, OR4N4, LOC650137	L	L	L	G	-	G
20q11.21	1022.42	DEFB115-116,118-119,121,123-124, REM1, HM13, ID1², COX4I2⁴, BCL2L1²	-	-	G	-	-	-
22q13.2	17.66	SCUBE1¹	-	-	-	-	-	L

Recurrent genomic variations observed during hESC lines H1 and H9 in vitro culture using manual or enzymatic techniques. Chromosomal region, minimal/maximal sizes, common genes and variation type (loss/gain) are mentioned. E, enzymatic passages; G, gain; L, loss; M, manual passages; max, maximal; min, minimal; ¹, genes related to development; ², genes related to cell cycle, growth and apoptosis; ³, genes related to tumorigenesis; ⁴, genes related to syndrome and disease.

ISSN: 1755-8166