Redistribution of PO41 RNA during the cell cycle. DNA/RNA FISH with the PO41neg probe (red) representing the redistribution of PO41 RNA during the cell division. MDCC-MSB1 cells at interphase (a), prophase (b), metaphase (c, d), anaphase (e, f), telophase (g, h) and cytokinesis (i, i’) are shown. Images of anaphase and telophase cells were acquired with higher gain values. 3D reconstructions of MDCC-MSB1 cells at anaphase (e’) and telophase (g’) stages with isosurfaces around G-rich transcripts of PO41 repeat (red) and chromatin (blue). DNA was counterstained with DAPI. Scale bars: 3 μm (a-i, e’); 2 μm (g’).