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Association of microRNA-146a and their target gene IRAK-1 polymorphism with enthesitis related arthritis category of juvenile idiopathic arthritis

Molecular Cytogenetics20147(Suppl 1):P69

https://doi.org/10.1186/1755-8166-7-S1-P69

Published: 21 January 2014

Keywords

  • MicroRNAs
  • miR-146a
  • rs2910164
  • Polymorphisms
  • JIA-ERA

Background

MicroRNAs (miRNAs) are non-coding RNA molecules that play pivotal role in modulating the expression of multiple target genes at the post-transcriptional level. Single Nucleotide Polymorphisms (SNP) in pre-miRNAs can alter miRNA expression, and polymorphism in target molecules can affect binding to target mRNA. Studies have shown an association between miR-146a polymorphisms and autoimmune disease. Taking into account that interleukin-1 receptor-associated kinase-1 (IRAK-1) is a target of miR-146a, we studied the association between SNPs of miRNA-146 and its target IRAK-1 with susceptibility to Juvenile Idiopathic Arthritis-Enthesitis Related Arthritis (JIA-ERA).

Methods

One hundred and fifty patients with JIA-ERA (ILAR criteria) were included in the study. 216 blood donors (201 male) with a mean age of 30.5 years served as controls. miR-146a (C/G) (rs2910164) and it’s target IRAK-1(C/T) (rs1059703) at Exon 12 region and IRAK-1 (A/C) (rs3027898) at 3’UTR polymorphisms were analyzed using PCR-RFLP method.

Results

Among 150 patients, 134 were males and the mean age at onset of disease was 11 (4-16) years, mean disease duration was 4.5 (0.3-12) years. 22 had uveitis and 21 had positive family history. 73 had enthesitis and 75 had inflammatory back pain and all had arthritis. 116 were HLA B27 positive.

Genotype frequency of miR-146a gene was in Hardy Weinberg equilibrium in healthy controls whereas in IRAK-1 genotype the frequency was contrary owing to its presence on chromosome X.

The genotype frequency for miR-146a were different in controls and patients [GG (51.85% vs 50.0%), GC (42.13% vs 37.29%) and CC (6.02% vs 12.71%), OR = 2.18; 95% CI 1.02 – 4.68; p value = 0.0418]

IRAK-1 (rs1059703) allelic frequencies in controls and patients were similar [CC (33.8% vs 32.9%), and TT (66.2% vs 67.0%)]. IRAK-1 (rs3027898) allelic frequencies were also similar among control and patients [CC (70.1% vs 76.1%) and AA (29.8% vs 23.9%)].

Conclusion

The CC genotype of the miR-146a rs2910164 polymorphism was significantly associated with the susceptibility to JIA-ERA.

Authors’ Affiliations

(1)
Department of Clinical Immunology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India
(2)
Department of Molecular and Human Genetics, Faculty of Science, Banaras Hindu University, Varanasi, India

Copyright

© Singh et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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