Table 1 Condition of metaphase arresting and enzyme treatment

Allium cepa, Allium fistulosum, Allium schoenoprasum, Allium altaicum

0.75 mM hydroxyurea for 20 h (RT), 0.05% colchicine for 3.5-4 h (RT)

0.6%

90–100 min

Humulus japonicus, Humulus lupulus, Linum usitatissimum, Cannabis sativa, Ricinus communis

2 mM 8-hydroxyquinoline, 4 h

0.6%

100–120 min

Triticale, Triticum aestivum, Thinopyrum ponticum, Thinopyrum elongatum

0.2% colchicine, 2 h¹

1.2%

100–120 min

Brassica nigra, Brassica oleracea,

1-bromnaphtalene (1:1000 water solution) overnight 4°C

0.6%

90–100 min

Aloe vera,

1.2%

100–120 min

Rosa wichurana

0.3%

90–120 min

Anthurium andreanum, Monstera deliciosa, Philodendron scandens, Spathiphyllum wallisii, Syngonium auritum, Zantedeschia elliotiana, Hippophae rhamnoides, Festuca arundinacea and Lolium perenne

0.1%

60–90 min

Rosa wichurana ²

Mix of 0.1% colchicine and 2 mM 8-hydroxyquinoline (4 h, RT)³

1.2%

120–150 min

Allium wakegi, Allium roylei

Nitrous oxide gas (1.0 MPa), 3 h

0.6%

90–100 min

Allium cepa PMC

-

1.5%

180–200 min