The accuracy of chromosomal microarray testing for identification of embryonic mosaicism in human blastocysts

Table 2 Follow-up FISH data for embryos diagnosed with mosaic aneuploidy by CMA

Specimen designation	TE cell #	CMA aneuploidy^a	Log₂ratio	Abnormal nuclei by FISH %^b	Non-mosaic^c	Mosaic^d	Embryo confirmed abnormal^e
1 M	2	+14	0.193	97	Yes	No	Yes
2 M	4	+8	0.206	53	No	Yes	Yes
3 M	3	-1	-0.155	10	No	Yes	Yes
4 M	5	-4	-0.235	21	No	Yes	Yes
5 M	4	-19	-0.173	20	No	Yes	Yes
6 M	4	-4	-0.154	10	No	Yes	Yes
7 M	4	+5	0.220	27	No	Yes	Yes
7 M	4	+10	0.133	6	No	No	Yes
8 M	4	-8	-0.203	20	No	Yes	Yes
9 M	3	-14	-0.178	12	No	Yes	Yes
10 M	2	-9	-0.183	7	No	No	No
11 M	4	+15	0.230	63	No	Yes	Yes
11 M	4	+17	0.164	20	No	Yes	Yes
12 M	11	-8	-0.280	19	No	Yes	Yes
12 M	11	-11	-0.141	28	No	Yes	Yes
13 M	7	-8	-0.230	71	No	Yes	Yes
14 M	4	+2	0.211	40	No	Yes	Yes
14 M	4	+8	0.181	26	No	Yes	Yes
15 M	4	+22	0.136	16	No	Yes	Yes
16 M	4	+1	0.168	15	No	Yes	Yes
17 M	4	-18	-0.216	58	No	Yes	Yes
18 M	4	-8	-0.199	43	No	Yes	Yes
18 M	4	+16	0.179	30	No	Yes	Yes
19 M	2	-3	-0.146	23	No	Yes	Yes
19 M	2	+X	0.159	19	No	Yes	Yes
20 M	4	+19	0.159	23	No	Yes	Yes
20 M	4	+X	0.149	26	No	Yes	Yes
21 M	3-4	-19	-0.162	56	No	Yes	Yes

^aEmbryos listed in this table were identified by CMA to have at least one mosaic chromosome aneuploidy.
^bA nucleus is considered abnormal by FISH if there is a loss or gain of the chromosome identified by CMA to be aneuploid.
^cA non-mosaic CMA result is considered concordant with FISH if ≥ 90% of follow-up embryo cells have a FISH signal pattern consistent with the CMA data.
^dA mosaic CMA result is considered concordant with FISH if ≥ 10% and < 90% of follow-up embryo cells have a FISH signal pattern consistent with the CMA data.
^eAn embryo follow-up result is considered abnormal and concordant with an abnormal CMA result if at least one chromosome in an embryo identified by CMA as abnormal is confirmed by FISH.

ISSN: 1755-8166