The accuracy of chromosomal microarray testing for identification of embryonic mosaicism in human blastocysts

Table 1 Follow-up FISH data for embryos diagnosed with non-mosaic aneuploidy by CMA

Specimen designation	TE cell #	CMA aneuploidy^a	Log₂ratio	Abnormal nuclei by FISH (%)^b	Non-mosaic^c	Mosaic^d	Embryo confirmed abnormal^e
1 NM	6	-X	-0.501	100	Yes	No	Yes
1 NM	6	-13	-0.461	100	Yes	No	Yes
2 NM	3	+15	0.284	84	No	Yes	Yes
3 NM	2	-X	-0.721	37	No	Yes	Yes
		+Y	0.440	47	No	Yes
		+21	0.369	47	No	Yes
4 NM	2	-13	-0.409	21	No	Yes	Yes
4 NM	2	-18	-0.446	47	No	Yes	Yes
5 NM	6	-22	-0.430	100	Yes	No	Yes
6 NM	5	-6	-0.487	100	Yes	No	Yes
7 NM	7	-14	-0.374	94	Yes	No	Yes
8 NM	7	+15	0.300	94	Yes	No	Yes
9 NM	2	-Y	-0.237	0	No	No	Yes
9 NM	2	+21	0.465	92	Yes	No	Yes
10 NM	5	+15	0.317	97	Yes	No	Yes
11 NM	2	-15	-0.508	74	No	Yes	Yes
12 NM	4	-19	-0.382	91	Yes	No	Yes
13 NM	3	+5	0.294	80	No	Yes	Yes
		+14	0.249	83	No	Yes
		-18	-0.529	100	Yes	No
14 NM	3	-14	-0.555	73	No	Yes	Yes
15 NM	1	-22	-0.395	94	Yes	No	Yes
16 NM	4	-8	-0.642	97	Yes	No	Yes
16 NM	4	+11	0.336	97	Yes	No	Yes
17 NM	3	-21	-0.432	73	No	Yes	Yes
18 NM	2	+9	0.375	94	Yes	No	Yes
19 NM	5	+16	0.253	86	No	Yes	Yes
20 NM	4	-22	-0.407	87	No	Yes	Yes
21 NM	3	-4	-0.440	12	No	Yes	Yes
21 NM	3	-10	-1.540	0	No	No	Yes
22 NM	1-2	-X	-0.485	87	No	Yes	Yes
22 NM	1-2	+13	0.398	71	No	Yes	Yes
23 NM	4	+8	0.288	94	Yes	No	Yes
23 NM	4	+22	0.268	97	Yes	No	Yes
24 NM	8	-19	-0.417	80	No	Yes	Yes
25 NM	3	+22	+0.256	86	No	Yes	Yes
26 NM	3	-10	-0.561	94	Yes	No	Yes

^aAll chromosomes listed as abnormal in the embryos of this table were identified by BlueFuse software to be aneuploid.
^bA nucleus is considered abnormal by FISH if there is a loss or gain of the chromosome identified by CMA to be aneuploid.
^cA non-mosaic CMA result is considered concordant with FISH if ≥ 90% of follow-up embryo cells have a FISH signal pattern consistent with the CMA data.
^dA mosaic CMA result is considered concordant with FISH if ≥ 10% and < 90% of follow-up embryo cells have a FISH signal pattern consistent with the CMA data.
^eAn embryo follow-up result is considered abnormal and concordant with an abnormal CMA result if at least one chromosome in an embryo identified by CMA as abnormal is confirmed by FISH.

ISSN: 1755-8166