FISH analysis. The chromosomes involved in the dic(9;13)(pter→p11.2::p12→qter),neo(9)(pter→p12→neo→p11.2) translocation: examples of FISH results (details in Additional file 1: Table S1). A. FISH results for 4 probes: centromere 9 probe (red), 9p subtelomere (red), 9q subtelomere (green) and centromere 13/21 (green). B. FISH results for 3 probes: centromere 9 (yellow), 9q subtelomere (green) and 13q subtelomere (red). C. FISH results for painting probes: 9 wcp (green) and 13 wcp (red). D. FISH results for 3 probes: 9 centromere (red), 9p11.2 - specific RP11-259A5 (red) and 13/21 centromere probe (green) showing that chromosome 9 region 9p11.2 is present on the normal and dic(9;13) chromosomes. E. Tricolour FISH results with chromosome 9 (red) and 13/21 (green) centromeric probes shows the presence of these probes on dic(9;13) and each of the normal chromosomes. The yellow probe RP11-318 K12 corresponds to the 9q13 region and is present on the dic(9;13) chromosome and the normal chromosome 9q12.