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Table 1 Probes and libraries used for FISH

From: Chromosome anomalies in bone marrow as primary cause of aplastic or hypoplastic conditions and peripheral cytopenia: disorders due to secondary impairment of RUNX1 and MPL genes

Pt Chromosome Probes/libraries Localization/Sequences recognized
1 13 RP11-1001I7a 13q14.2
  21 WCP 21b whole chromosome paint library
   21qtel07c subtelomeric region
   AML1/ETOd designed for t(8;21)
   RP11-79G23a 21q21.3
   RP11-203G22a 21q22.12
   CTD-2349F18a RUNX1 gene, intron1-exon2
   CTD-2235K24a RUNX1 exons 2–8
   RP11-625E21a 21q22.2
   RP11-88N2a 21q22.3
2 21 WCP 21b whole chromosome paint library
   21qtel07c subtelomeric region
   AML1/ETOd designed for t(8;21)
   CTD-2532E17a 21p11.2-11.1
   RP11-468N22a 21q22.11-22.12
   WI2-1915K14e RUNX1 intron 1
   CTD-2349F18a RUNX1 intron1-exon2
   CTD-2235K24a RUNX1 exons 2–8
   WI2-942D2e RUNX1 exons 2–4
   WI2-605D9e RUNX1 exons 2–5
   WI2-847D7e RUNX1 exon 6
   WI2-542L2e RUNX1 exons 7-8
3 1 RP11-467K11a 1p36.32
   RP11-372C15a 1p36.31
   RP11-690E2a 1p36.23
   RP11-113C10a, RP11-90B12a 1p34.2, flanking the MPL gene
   RP11-46G23a, RP11-125P23a 1p12
   RP11-206H22a 1p11.2
  1. a BAC probes, Invitrogen Corporation, Carlsbad, CA, USA.
  2. b WCP, Cytocell Technologies, Cambridge, UK.
  3. c 21 qtel07, Cytocell Technologies, Cambridge, UK.
  4. d LSI AML1/ETO dual colour dual fusion translocation probe, Abbott, Abbott Park, IL, USA.
  5. e Fosmid probes, kindly provided by Prof. Peter De-Jong, BACPAC Resources Center, Childrens Hospital Oakland Research Institute, Oakland, CA, USA.
  6. Patients (Pt), chromosome localization and sequences related to the genes of interest recognized.