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Table 1 Probes and libraries used for FISH

From: Chromosome anomalies in bone marrow as primary cause of aplastic or hypoplastic conditions and peripheral cytopenia: disorders due to secondary impairment of RUNX1 and MPL genes

Pt

Chromosome

Probes/libraries

Localization/Sequences recognized

1

13

RP11-1001I7a

13q14.2

 

21

WCP 21b

whole chromosome paint library

  

21qtel07c

subtelomeric region

  

AML1/ETOd

designed for t(8;21)

  

RP11-79G23a

21q21.3

  

RP11-203G22a

21q22.12

  

CTD-2349F18a

RUNX1 gene, intron1-exon2

  

CTD-2235K24a

RUNX1 exons 2–8

  

RP11-625E21a

21q22.2

  

RP11-88N2a

21q22.3

2

21

WCP 21b

whole chromosome paint library

  

21qtel07c

subtelomeric region

  

AML1/ETOd

designed for t(8;21)

  

CTD-2532E17a

21p11.2-11.1

  

RP11-468N22a

21q22.11-22.12

  

WI2-1915K14e

RUNX1 intron 1

  

CTD-2349F18a

RUNX1 intron1-exon2

  

CTD-2235K24a

RUNX1 exons 2–8

  

WI2-942D2e

RUNX1 exons 2–4

  

WI2-605D9e

RUNX1 exons 2–5

  

WI2-847D7e

RUNX1 exon 6

  

WI2-542L2e

RUNX1 exons 7-8

3

1

RP11-467K11a

1p36.32

  

RP11-372C15a

1p36.31

  

RP11-690E2a

1p36.23

  

RP11-113C10a, RP11-90B12a

1p34.2, flanking the MPL gene

  

RP11-46G23a, RP11-125P23a

1p12

  

RP11-206H22a

1p11.2

  1. a BAC probes, Invitrogen Corporation, Carlsbad, CA, USA.
  2. b WCP, Cytocell Technologies, Cambridge, UK.
  3. c 21 qtel07, Cytocell Technologies, Cambridge, UK.
  4. d LSI AML1/ETO dual colour dual fusion translocation probe, Abbott, Abbott Park, IL, USA.
  5. e Fosmid probes, kindly provided by Prof. Peter De-Jong, BACPAC Resources Center, Childrens Hospital Oakland Research Institute, Oakland, CA, USA.
  6. Patients (Pt), chromosome localization and sequences related to the genes of interest recognized.