Table 1 Methods and results of immunohistochemistry
Antibody | Source | Dilution/antigen retrieval | Detection method | Reactivity |
|---|---|---|---|---|
CK AE1/AE3 | Dako | 1:200/protease digestion | SA/Bi | - |
CK7 | Dako | 1:200/protease digestion | SA/Bi | - |
CK20 | Dako | 1:500/protease digestion | SA/Bi | - |
TTF1 | Dako | 1:100/CC1/95°C/30 min | SA/Bi | - |
EMA | Dako | 1:200/CC1/95°C/30 min | SA/Bi | - |
Vimentin | Biogenex | 1:10000/CC1/95°C/30 min | SA/Bi | - |
CD20 | Dako | 1:250/CC1/95°C/30 min | SA/Bi | - |
CD30 | Dako | 1:50/CC1/95°C/30 min | SA/Bi | - |
CD34 | Cell Marque | 1:50/CC1/95°C/30 min | SA/Bi | - |
Kappa light chain | Dako | 1:5000/protease digestion | SA/Bi | - |
Lambda light chain | Dako | 1:10000/protease digestion | SA/Bi | - |
CD68 | Dako | 1:800/CC1/95°C/8 min | SA/Bi | - |
S-100 | Univ. of Toronto | 1:1000/CC1/95°C/30 min | SA/Bi | - * |
HMB-45 | Dako | 1:100/untreated | SA/Bi | - |
Melan-A | Cell Marque | 1:50/CC1/95°C/30 min | SA/Bi | - * |
PNL2 | Private source | RTU/untreated | Polymer based | - |
SMA | Dako | 1:200/untreated | SA/Bi | - |
Desmin | Dako | 1:200/CC1/95°C/30 min | SA/Bi | +++ |
H-Caldesmon | Dako | 1:200/CC1/95°C/30 min | SA/Bi | - |
Myogenin | Dako | 1:50/CC1/95°C/60 min | Polymer based | - |
Myo-D1 | Dako | 1:60/citrate buffer (pH6.0) | Polymer based | - |
CD99 | Signet | 1:20/CC1/95°C/30 min | SA/Bi | +++ |
Synaptophysin | Cell Marque | 1:250/CC1/95°C/30 min | SA/Bi | +++ |
Fli1 | BD Pharmingen | 1:20/CC1/95°C/60 min | Polymer based | - |
WT1 | Dako | 1:50/CC1/95°C/60 min | Polymer based | - |
Ki 67 | Lab Vision | 1:200/CC1/95°C/30 min | SA/Bi | >10% |