Fig. 8

NGS reads from alpaca flow karyotype are shown as in Fig. 6—however, here chromosome-specific views are provided in reference coverage charts. (A) VGA peak C is distributed across the human X chromosome. Short to long arm is from left to right. The lack of signals at the distal short arm may correspond to a lack of homology with the human XY pseudo-autosomal (pairing) region. The gap about one third along to the right may be the site of the X centromere. (B) VPA peaks A (B-1) and L (B-2) aligned to reference human chromosome 3 show that the short arm of HSA 3 has homology to peak L and the long arm to VPA peak A, corresponding to CDR 1 and CDR 17 respectively. (C) VPA peak D show homology to most of human chromosomes 14 (C-1) and 15 (C-2) except for the short arms (left) of both human acrocentric chromosomes, which carry ribosomal and centromeric repeats. HSA 14 and 15 are syntenic in most mammalian karyotypes and are both present in CDR 4. (D) VPA peaks G (D-1) and K (D-2) are distributed across human chromosome 6 and map to CDR 18 and 20. The breakpoint appears distinct in HSA 6p but not in HSA 6q. (E) VPA peaks H (E-1) and O (E-2) are distributed across the long and short arms respectively of human chromosome 16. These regions share homology with CDR 9 and 18. The large gap between the two may in part be due to the large heterochromatic region of HSA proximal 16q. (F) VPA peaks H (F-1) and W (F-2) map to short regions of human chromosome 1 and share homology with short regions of CDR 9 and 21