Fig. 3From: The rare mutation in the endosome-associated recycling protein gene VPS50 is associated with human neural tube defectsCo-Immunoprecipitation illustrated the effect of novel mutations on the physical interaction of VPS50 with VPS53. HEK293t cells were co-transfected into 4μg VPS50 plasmid constructs in pairwise combinations with 4μg VPS53 cDNA. HA immunoprecipitation, blotting with anti-Flag confirmed physical interaction between WT VPS50 and VPS53. This interaction was decreased in the p. Gly169Val mutationBack to article page