Table 1 Primer for MS-PCR

p15 [22]:

(methylated, 148 bp, 60 °C)

forward:

5′-GCGTTCGTATTTTGCGGTT-3′

reverse:

5′-CGTACAATAACCGAACGACCGA-3′

(unmethylated, 154 bp, 60 °C)

forward:

5′-TGTGATGTGTTTGTATTTTGTGGTT-3′

reverse:

5′-CCATACAATAACCAAACAACCAA-3′

p16 [23]:

(methylated 150 bp, 65 °C)

forward:

5′-TTATTAGAGGGTGGGGCGGATCGC-3′

reverse:

5′-GACCCCGAACCGCGACCGTAA-3′

(unmethylated 151 bp, 65 °C)

forward:

5′-TTATTAGAGGGTGGGGTGGATTGT-3′

reverse:

5′-CAACCCCAAACCACAACCATAA-3′

MGMT [21]:

(methylated, 122 bp, 54 °C)

forward:

5′-GTTTTTAGAACGTTTTGCGTTTCGAC-3′

reverse:

5′-CACCGTCCCGAAAAAAAACTCCG-3′

(unmethylated, 129 bp, 56 °C)

forward:

5′-TGTGTTTTTAGAATGTTTTGTGTTTTGAT-3′

reverse:

5′-CTACCACCATCCCAAAAAAAAACTCCA-3′