Fig. 3
From: Active centromere and chromosome identification in fixed cell lines
Representative images of HEL metaphase for each method, with marked (i) der(9) and (ii) psu dic(22;9). a Standard mFISH. Metaphase spread (left) and karyotype (right) in false colour. b cenFISH-mFISH. Upper panel: immunofluorescence images showing α-satellite (green) and DAPI (blue); insets are enlarged images of (i) der(9) and (ii) psu dic(22;9). Lower panel: metaphase spread and karyotype in false colour. c CENP-IF-cenFISH-mFISH with CENP-A. Upper panel: immunofluorescence images of cytospun cells showing α-satellite (green), CENP-A (red) and DAPI (blue); insets are enlarged images of (i) der(9) and (ii) psu dic(22;9). Lower panel: metaphase spread and karyotype in false colour display. (iii) and (iv) are der(10;19) with two pairs of CENP-A signals. d CENP-IF-cenFISH-mFISH with CENP-C. Upper panel: immunofluorescence images showing α-satellite (green), CENP-C (red) and DAPI (blue). Lower panel: metaphase spread and karyotype in false colour display; (i) and (ii) are der(9) and psu dic(22;9) respectively while (iii) is der(10;19) and (iv) is der(20)t(11;15;20) (as named in MacKinnon et al. [11]) observed with two pairs of CENP-C signals