Table 2 I-FISH, MLPA and SNP array findings for two lesions of case no. 2
| Â | MLPA | MS-MLPA | I-FISH | SNP array | Treatment | |||
|---|---|---|---|---|---|---|---|---|
Gene | Location | Result | Gene | Result | ||||
First resection - anaplastic astrocytoma | IDH1 | 2q33.3 | R132H | MSH2 | normal | deletion of CDKN2A deletion of subtelomeric 16q | (X)x1, (2)x2hmz, 3p26.3p11.1(66,894-90,089,238)x2hmz, 4q12q13.1(52,728,324-66,300,425)x1, (5)x1,6p25.3p21.33(204,909-31,335,647)x1, 6q22.33q27(128,989,302-170,898,549)x1, 7p22.3p22.1(46,239-5,016,400) x1, 7q22.1q36.3(98,451,358-159,119,486)x3, 9p24.3p13.1(46,587-38,771,460)x1, 10q21.2q26(61,465,713-135,430,043)x1, 11p15.5p11.2(203,788-47,430,599)x2hmz, 11p11.2 (47,801,101-51,274,692)x1, 12q12q24.33(38,416,139-133,429,634) x2hmz, 13q12.11q14.2(19,888,741-50,144,613)x2hmz, 13q14.2q21.33(50,283,115-69,338,113)cth, 14q11.2q22.2(20,213,937-54,316,607)x2hmz, 14q23.2q32,12(62,420,606-93,152,220)x1, 15q21.1q24.1(46,805,269-74,506,930)x1, (16)x2hmz, 17p13.3p11.2(18,901-20,219,226) x2hmz, 17q25.3(79,894,097-81,047,565)x1, 18q23(76,324,504-78,014,582)x1, 19p13.3 (267,039-1,569,839)x3, (21)x2hmz, (22)x2hmz | Chemotherapy |
CRBN | 3p26.3 | normal | ||||||
CRELD1 | 3p25.3 | normal | ||||||
VHL | 3p25.3 | normal | MSH6 | normal | ||||
PPARG | 3p25.1 | normal | ||||||
RAF1 | 3p25.1 | normal | ||||||
HESX1 | 3p14.3 | normal | MLH1 | normal | ||||
LFNG | 7p22.2 | del | ||||||
FKBP9 | 7p14.3 | normal | ||||||
GLI3 | 7p14.1 | normal | MSH3 | normal | ||||
ADAM22 | 7q21.12 | normal | ||||||
TFR2 | 7q22.1 | normal | ||||||
KIAA1549 | 7q34 | amp | PMS2 | normal | ||||
HIPK2 | 7q34 | amp | ||||||
MKRN1 | 7q34 | amp | ||||||
BRAF | 7q34 | amp | MGMT | methylated (19Â %) | ||||
DPP6 | 7q36.2 | amp | ||||||
MIR31 | 9p21.3 | del | ||||||
CDKN2A | 9p21.3 | del | MLH3 | normal | ||||
CDKN2B | 9p21.3 | del | ||||||
IDH2 | 15q26.1 | normal | ||||||
Second resection - anaplastic astrocytoma | IDH1 | 2q33.3 | R132H | MSH2 | normal | deletion of CDKN2A deletion of PTEN | (X)x1, 1q41q43(216,318,021-241,388,129) x2hmz, 2q14.2q22.3(120,617,488-144,598,432) x2hmz, 4q12q13.1 (52,728,324-66,300,425)x1, (5)x1,6p25.3p21.33 (204,909-31,335,647)x1, 6q22.33q27(128,989,302-170,898,549)x1, 7p22.3p22.1 (46,239-5,016,400)x1, 7q22.1q36.3(98,451,358-159,119,486)x3, 9p24.3p13.1 (46,587-38,771,460)x1, 10q21.2q26(61,465,713-135,430,043)x1, 11p15.5p11.2 (203,788-47,430,599)x2hmz, 11p11.2(47,801,101-51,274,692)x1, 13q14.2q21.33 (50,283,115-69,338,113)cth, 14q23.2q32,12 (62,420,606-93,152,220)x1, (16)x2hmz, 17p13.3p11.2(18,901-20,219,226)x2hmz, 17q25.3(79,894,097-81,047,565)x1, 18q23(76,324,504-78,014,582)x1, 19p13.3(267,039-1,569,839)x3, (22)x2hmz | Chemotherapy |
CRBN | 3p26.3 | normal | ||||||
CRELD1 | 3p25.3 | normal | ||||||
VHL | 3p25.3 | normal | MSH6 | normal | ||||
PPARG | 3p25.1 | normal | ||||||
RAF1 | 3p25.1 | normal | ||||||
HESX1 | 3p14.3 | normal | MLH1 | normal | ||||
LFNG | 7p22.2 | del | ||||||
FKBP9 | 7p14.3 | normal | ||||||
GLI3 | 7p14.1 | normal | MSH3 | normal | ||||
ADAM22 | 7q21.12 | normal | ||||||
TFR2 | 7q22.1 | normal | ||||||
KIAA1549 | 7q34 | amp | PMS2 | normal | ||||
HIPK2 | 7q34 | amp | ||||||
MKRN1 | 7q34 | amp | ||||||
BRAF | 7q34 | amp | MGMT | methylated (25Â %) | ||||
DPP6 | 7q36.2 | amp | ||||||
MIR31 | 9p21.3 | del | ||||||
CDKN2A | 9p21.3 | del | MLH3 | methylated | ||||
CDKN2B | 9p21.3 | del | ||||||
IDH2 | 15q26.1 | normal | ||||||