Figure 4

Fluorescent in situ hybridisation analysis Cytochalasin B treated mononuclear cells from Case no. 1. A) FISH image showing multiple chromosomes contained in the nuclear bulge using an all chromosome alpha satellite probe (green). B) two signals specific to the 1p arm (orange) and one signal specific to the 1q arm (green) present in the nuclear bulge while the larger nucleus showed four signal copies for 1q and two signal copies for 1p that correspond to the karyotype in Figure 1A (left image. C) FISH studies on binucleate daughter cells using an all chromosome alpha satellite FISH probe (green) showing expulsion of chromosome material from the binucleate cell to the micronucleus. D) FISH signal pattern for 1p and 1q arm probes showing unequal signal distribution of 1p and 1q in the two daughter nuclei and in the micronucleus.