Genomic amplification of BCR/ABL1 and a region downstream of ABL1 in chronic myeloid leukaemia: a FISH mapping study of CML patients and cell lines

Table 2 Summary of the FISH screening results with the probes RP11-83J21 (band 9q34.12) and RP11-323H21 (band 9q34.13)

ID	Cell clones identified by metaphase FISH analysis		Isoderivative Ph?	High copy number gains in markers ?
1	i.	t(9;22)[1]	No	No
	ii.	t(9;22),+der(22)[19]
2	i.	t(9;22)[18]	No	No
	ii.	t(9;22),+der(22)[2]
3	i.	t(9;22),+der(22)[20]	No	No
4	i.	t(9;22),+der(22)[20]	No	No
5	i.	t(9;22),+der(22)[20]	No	No
6	i.	t(9;22)[7]	No	No
	ii.	t(9;22),+der(22)[13]
7	i.	t(9;22)[10]	No	No
	ii.	t(9;22),+der(22)[10]
8	i.	No t(9;22)[12]	No	No
	ii.	t(9;22),del(9),+der(22)[8]¹
9	i.	t(9;22)[17]	No	No
	ii.	t(9;22),+der(22)[3]
10	i.	No t(9;22)[6]	No	No
	ii.	t(9;22)[1]
	iii.	t(9;22),+der(22)[13]
11	i.	t(1;22;9)[1]	No	No
	ii.	t(1;22;9),+der(22)t(1;22;9)[19]²
12	i.	t(9;22),+der(22)[6]³	No	No
13	i.	t(9;22)[6]	Yes	No
	ii.	t(9;22),del(9) [12]¹
	iii.	t(9;22),del(9),+der(22)[2]¹
	iv.	t(9;22),del(9),ider(22)[1]¹
14	i.	No t(9;22)[10]	Yes	No
	ii.	t(9;22)[4]
	iii.	t(9;22),ider(22)[1]
15	i.	t(9;22)[4]	Yes	No
	ii.	t(9;22),+der(22)[12]
	iii.	t(9;22),ider(22)[1]
16	i.	t(9;22)[8]	Yes	No
	ii.	t(9;22),+der(22)[10]
	iii.	t(9;22),ider(22),+ider(22)[1]
17	i.	No t(9;22)[7]	Yes	No
	ii.	t(9;22)[3]
	iii.	t(9;22),+der(22)[8]
	iv.	t(9;22),ider(22)[1]⁴
18	i.	No t(9;22)[13]	No	Yes
	ii.	t(9;22),marker[1]
19	i.	t(9;22)[1]	Yes	Yes
	ii.	t(9;22),+der(22)[15]
	iii.	t(9;22),ider(22),marker[2]
	iv.	t(9;22),marker[8]
	v.	t(9;22),marker,marker[8]

¹Deletion on the non-translocated homologue of chromosome 9.² BCR/ABL1 fusion on derivative 1.³ FISH analysis on interphase cells showed a ratio of normal cells versus BCR/ABL1 positive cells (with an extra fusion signal) of 95% vs 5%.⁴ Further translocation of the isoderivative 22 with a marker chromosome.

ISSN: 1755-8166