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Figure 5 | Molecular Cytogenetics

Figure 5

From: Genomic amplification of BCR/ABL1 and a region downstream of ABL1 in chronic myeloid leukaemia: a FISH mapping study of CML patients and cell lines

Figure 5

Gains of BCR/ABL1 fusion and a 438 Kb region from 9q34 in the cell line K562. (A) A representative metaphase cell and interphase cell with co-hybridization of FISH probes RP11-83J21 (3' ABL1; signal in red) and RP11-544A12 (NUP214; in green) showing two acrocentric markers der(22)t(9;13;22)(q34;q11;q11) (indicated by block arrows) with multiple fusion signals, plus two fusion on a der(9)t(9;9) and one fusion signal on a der(9)t(9;17). (B) A representative metaphase cell and interphase cell with co-hybridization of FISH probes RP11-643E14 (in red) and RP11-323H21 (5' RAPGEF1; in green) showing just two fusion signals on der(9)t(9;9) and one on der(9)t(9;17), with no hybridization on the markers with BCR/ABL1 amplification. The distal breakpoint of the amplicon falls between RP11-544A12 and RP11-643E14 and the amplified region from 9q34 is estimated to be 438 Kb long. (C) Representative metaphase cell with co-hybridization of 9q and 22q sub-telomeric FISH probes (in red and green, respectively) showing two signals from 22qter on two chromosomes 22 and three signals from 9qter on both arms of der(9)t(9;9) and on der(9)t(9;17).

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Molecular Cytogenetics

ISSN: 1755-8166