Figure 3

High copy number gains of BCR/ABL1 fusion and downstream region in two CML patients. (A) Representative interphase cells from patient no. 18 with co-hybridization of FISH probes RP11-83J21 (signal in red) and RP11-323H21 (in green). Left, a cluster of fusion signals was detected in 29% of cells. Right, 71% of cells showed only two signals corresponding to both normal homologues of chromosome 9. (B-F) Representative metaphase cells from the cell clones found in patient no. 19, indicating the number of fusion signals and the percentage of cells with that signal pattern. 3% of the metaphase cells had one Ph chromosome (B); 44% had two Ph (C); 6% had an isoderivative 22 Ph and a fourth fusion signal on a marker chromosome (D); 23.5% had one Ph, one marker with tandem repeats of the 3'ABL1-RAPGEF1 region (1.09 Mb) and one marker with duplication of the 3'ABL1-NUP214 region (682 Kb) (E); 23.5% cells had one Ph and one marker with high copy number gains of the 3'ABL1-NUP214 region (682 Kb) (F).