From: Human interphase chromosomes: a review of available molecular cytogenetic technologies
Technique | Brief description | Advantages | Limitations | Refs |
---|---|---|---|---|
I-FISH with centromeric probes | I-FISH on interpahse nuclei painting pericentromeric (heterochromatic) regions | High hybridization efficiency, chromosome specifity (apart few chromosomes) | Signal associations, impossible to analyze chromosomes 5,13, 14, 19, 21, 22; heteromorphisms | |
I-FISH with site-specific probes | I-FISH painting specific euchromatic genomic loci | Small specific genomic loci are visualized | Low hybridization efficiency, numerous artifacts | |
I-FISH with wcp | I-FISH painting chromosome territories | Identification of nuclear chromosome territories | Chromosome territories are ambiguous, no additional information | |
mFISH | Multicolor I-FISH with >2 probes labeled by different fluorochromes/ligands | Analysis of several targeted genomic loci | Difficulty to distinguish between artifacts and aneuploidy/polyploidy | |
mFISH/QFISH | mFISH + QFISH digitalization of FISH signals | Distinguishes between FISH artifacts and aneuploidy (polyploidy) | Same as mFISH | |
MFISH | Simultaneous visualization of the complete set of chromosomes in an interphase nucleus | All chromosome territories are simultaneously seen | Exceedingly sophisticated analysis; data poorly interpretable | |
ICS-MCB | Chromosome-specific MCB generated on interphase nuclei | Visualization of whole banded interphase chromosomes in their integrity | A pair of homologous chromosomes is studied per assay; relative complexity of the analysis |