Fig. 3

5q inversion and deletion. A G-banded normal chromosome 5 and der(5)del(q15q32)inv(p13q32). B Metaphase FISH using telomere (5p—green/5q—red) probes (Abbott Molecular) on inverted DAPI-banded chromosomes (normal and abnormal). C Metaphase FISH using 5q31 (red)/D5S23/D5S721 (green) probe (Abbott Molecular) on inverted DAPI-banded chromosomes (normal and abnormal). D Metaphase FISH using PDGFRβ break apart probe (Cytocell); 5′(green)/3′(red). Loss of 3′ centromeric probe (red) shown on inverted DAPI-banded abnormal derivative chromosome 5. E Ideogram of normal 5 (a) and der(5) (b) showing excision of a large segment of DNA located on 5q15q32 (red), followed by a large pericentric inversion inv(5)(p13q32) (yellow)