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Fig. 1 | Molecular Cytogenetics

Fig. 1

From: Molecular combing and its application in clinical settings

Fig. 1

Scheme of molecular combing technique (MCT): 0.7 to 1 million of cells (either from cell culture or from peripheral blood lymphocytes) must be included in the experiment. Cells are collected and included in an agarose plug from which high molecular weight DNA is extracted. The latter can be applied for MCT itself and coverslips with DNA-fibers are produced. Coverslips with DNA-fibers (Fig. 2) can be used in standard FISH and obtained results can be evaluated using a fluorescence microscope

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