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Table 3 Percentage of CIN in near-haploid HAP1 cell lines

From: Chromosomal instability (CIN) in HAP1 cell lines revealed by multiplex fluorescence in situ hybridisation (M-FISH)

Near haploid cell line ID and number of metaphases karyotyped

Percentage of N- and S-CIN in [1n] metaphases (%)

Percentage of N- and S-CIN in [2n] metaphases (%)

Total number of N- and S-CIN in [1n] and [2n] metaphases

Number of passages in cell culture

CRISPR-Cas9 gene editing

HAP1 Pool Lig4-Cas9[30]

None

7

7%[2]*

 ~ 20

Yes

B3 HAP1 P17[30]

20

7

27%[8]*

 ~ 33

Yes

1 N HAP1 P19[30]

7

3

10%[6]*

 ~ 35

Yes

HAP1 A1[30]

17

3

20%[6]*

 ~ 30

Yes

HAP1 A2[30]

10

7

17%[5]*

 ~ 30

Yes

HAP1 A5[30]

10

3

13%[4]*

 ~ 30

Yes

HAP1 E5[30]

23

10

33%[10]*

 ~ 30

Yes

HAP1 F3[30]

17

13

30%[9]*

 ~ 30

Yes

HAP1 G2[30]

20

7

27%[8]*

 ~ 30

Yes

HAP1-HO-C[25]

16

None

16%[4]*

 ~ 15

Yes

HAP1 GR 5.10[25]

40

None

40%[10]*

Unknown

No

4C1 R1[50]

6

12

18%[9]*

Minimum 10

No

4C1 R2[50]

4

12

16%[8]*

Minimum 10

No

19C1 R1[50]

6

12

18%[9]*

Minimum 10

Yes; LIG4 KO

HAP1 Pool Cas9 + Blast[30]

20

30

50%[15]*

 ~ 30

Yes; LIG4 KO

C6 HAP1[30]

–

–

None

 ~ 30

Yes; LIG4 & p53 KO

HAP1 GR S2[25]

–

–

None

Unknown

Yes

  1. Percentage of N- & S-CIN in near-haploid cell lines and passage numbers
  2. *Number of metaphases with N- & S-CIN (n.b. percentages include chromosome break)