Table 2 HAP1 cell lines analysed in the study
Cell line ID and ploidy status | CRISPR-Cas9 gene editing | Number of passages in cell culture | Percentage of haploidy based on 100 metaphases (%) | Percentage of diploidy based on 100 metaphases (%) |
|---|---|---|---|---|
HAP1 Pool Lig4-Cas9- [1n] | Yes |  ~ 20 | 88 | 12 |
C6 HAP1 [1n] | Yes |  ~ 30 | 89 | 11 |
B3 HAP1 P17 [1n] | Yes |  ~ 33 | 85 | 15 |
1 N-HAP1-P19 [1n] | Yes |  ~ 35 | 85 | 15 |
HAP1 A1 [1n] | Yes |  ~ 30 | 96 | 4 |
HAP1 A2 [1n] | Yes |  ~ 30 | 88 | 2 |
HAP1 A5 [1n] | Yes |  ~ 30 | 92 | 8 |
HAP1 E5 [1n] | Yes |  ~ 30 | 81 | 19 |
HAP1 F3 [1n]* | Yes |  ~ 30 | 90 | 10 |
HAP1 G2 [1n] | Yes |  ~ 30 | 78 | 22 |
HAP1-HO-C [1n] | No |  ~ 15 | 92 | 8 |
HAP1 GR 5.10 [1n] | No | Unknown | 92 | 8 |
HAP1 GR S2 [1n] | No | Unknown | 96 | 4 |
4C1 R1 [1n] | Yes; LIG4 KO | Minimum 10 | 85 | 15 |
4C1 R2 [1n] | Yes; LIG4 KO | Minimum 10 | 93 | 7 |
19C1 R1 [1n] | Yes; LIG4 & p53 KO | MINIMUM 10 | 85 | 15 |
HAP1 Pool CAS9 + BLAST [1n] | Yes |  ~ 30 | 65 | 35 |
HAP1-2 N-C [2n] | Yes |  ~ 15 | – | 100 |
HAP1-P53-KO [2n] | Yes |  ~ 20 | – | 100 |