Fig. 1

Pathological results of kidney biopsy and bone marrow aspiration smear and results of FISH. A, B The result of kidney biopsy shows increased lymphocytes, indicating lymphoblastic lymphoma or leukemia with kidney involvement (A: HE staining; original magnification, ×400 and B: PAS staining; original magnification, ×400). C The result of Wright-Giemsa staining of the bone marrow smear sample is shown. Bone marrow hyperplasia is extremely active, with lymphoblasts accounting for 50.8% of cells before treatment (Wright-Giemsa staining; original magnification, ×1000). D Lymphoblasts were negative for myeloperoxidase (red arrow), suggesting the possible diagnosis of acute lymphocytic leukemia (POX staining; original magnification, ×1000). E Fluorescence in situ hybridization (FISH) using a ZNF384 break-apart probe shows ZNF384 rearrangement in the kidney biopsy sample. Recognizable split signals can be seen in the scattered individual cells. The white arrows represent a single red signal and a single green signal (1R1G), which are split signals. The yellow arrow represents a single yellow signal (1Y), which is a normal signal. 1R1G1Y is a positive signal model for ZNF384 rearrangement. F Describes the ZNF384 rearrangement (39%) in the bone marrow sample, indicating homology with the rearrangement of ZNF384 in the kidney biopsy sample. The two yellow arrows on the left represent two yellow signals, which are negative signals for ZNF384 rearrangement. The white arrows and the yellow arrows on the right are indicative of ZNF384 gene rearrangement (1R1G1Y)