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Fig. 1 | Molecular Cytogenetics

Fig. 1

From: Differentially accessible, single copy sequences form contiguous domains along metaphase chromosomes that are conserved among multiple tissues

Fig. 1

Regions of differential accessibility between human metaphase homologs detected by single copy probe FISH. T-lymphocyte metaphase cells hybridized with scFISH probes from SCAMP2_IVS7-IVS4 (3.002 kb; 15q24.1), ZNF385D_cen678130 (2.251 kb, 3p24.3) and FGF6_tel4492 (1.66 kb, 12p12.3) [top row, left to right] show differential hybridization intensity between homologs. Homolog with lowest probe fluorescence intensity and reduced accessibility is on the right. Arrows indicate expected probe target location on each homolog. Control scFISH probe 3.3_1p36 (3.354 kb; 1p36) shows similar fluorescence intensities (or equivalent accessibility [EA]) between homologous targets. Chromosomes were counterstained with DAPI. Probes were labelled with digoxigenin-11-dUTP and detected with Cy3-digoxin antibody. Cells were imaged using Metasystems Axioimager Z.2 epifluorescence microscope system with Metafer4 (V3.8.12) and Isis package (V5.3) imaging software. Images are presented in inverted gray scale

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Molecular Cytogenetics

ISSN: 1755-8166