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Fig. 1 | Molecular Cytogenetics

Fig. 1

From: Molecular cytogenetic characterization and fusarium head blight resistance of five wheat-Thinopyrum intermedium partial amphiploids

Fig. 1

GISH and mcFISH patterns from lines HS2-2, HS2-4 and HS2-5. a, c, e Th. bessarabicum (J) genomic DNA labeled with fluorescein-12-dUTP was used as a probe for green signals, and common wheat cultivar Chinese Spring as a blocker. b, d, f The synthetic oligo pAs1-1, pAs1-3, and AFA-4 were 5′ end-labelled with 6-carboxytetramethyl-rhodamine (TAMRA) for red signals. The synthetic oligo pSc119.2-1 and (GAA) 10 were 5′ end-labelled with 6-carboxyfluorescein (6-FAM) for green signals. White arrows show the J-genome chromosomes. Bar = 10 μm. a, b line HS2-2, c, d: line HS2-4, e, f line HS2-5

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