Fig. 1From: Chromosome Y as a marker for sex discrepancies in patients with organ transplants: a case reportAnalysis of Y chromosome presence in maternal circulation. a Normalized chromosome value (NCV) for sex chromosomes in singleton XX and XY pregnancies. Male pregnancies exhibit a linear behavior, acquiring higher NCV_Y values as foetal fraction (FF) increases. However, female pregnancies have NCV_Y values closer to 0 and present NCV_X values between − 4 and 4. Note that NCV_Y values detected in maternal circulation of a pregnant woman subjected to a bone marrow transplant are out of scale and are more typical of that expected for an adult male reference sample. b Representative micrographs of FISH analysis performed in nuclei isolated from the white blood cell fraction of maternal blood sample. Probes: CEP18-spectrum aqua, CEPX-spectrum green, and CEPY-spectrum red. Positive signals for the CEPY-spectrum red probe confirm presence of the Y chromosome in WBCBack to article page