Detailed molecular cytogenetic characterisation of the myeloid cell line U937 reveals the fate of homologous chromosomes and shows that centromere capture is a feature of genome instability

Table 2 Results of FISH for other derivative chromosomes

Chromosome band	Chromosome position (GRCh38)	Marker/clone	Chromosome
Chromosome band	Chromosome position (GRCh38)	Marker/clone	psu dic(3;1)	del(1)(q11)	der(5)t(1;5)	der(5)t(5;13)
1 centromere			+ (inactive^a)	+
5q11.1	5:50139424-50285096	RP11-185I4				(+)
5q11.1	5:50768911-50961846	RP11-317O24			+	+ (15/20)/- (5/20) metaphases
+ (69/100)/- (31/100) interphases
5q11.2	5:57029637-57212477	RP11-101B14			+	+ (15/20)/- (5/20) metaphases
5q11.2	5:58287078-58423150	RP11-313I12			+	+

Chromosome band	Chromosome position (GRCh38)	Marker/clone	Chromosome
Chromosome band	Chromosome position (GRCh38)	Marker/clone	Cytogenetically normal 7 (one copy)	Normal 7 (other 2 or 3 copies)	der(7)t(6;7)(q27;q21.12) or del(7)(q22.1q34)
7p15	7:24505360-24515863	RP11-343P21	++	+	+

Chromosome band	Chromosome position (GRCh38)	Marker/clone	Chromosome
Chromosome band	Chromosome position (GRCh38)	Marker/clone	Cytogenetically normal 8 (one copy)	Normal 8 (other 2 copies)
8p	8:475607-658638	RP11-800L13	++	+

Each + represents a signal intensity equivalent to one copy on the normal chromosome; ++ represents a double-strength signal; (+) represents reduced intensity signal
^aAs inferred by the absence of a centromeric constriction

ISSN: 1755-8166