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Table 2 Morphology and flow cytometry results

From: Coexistence of recurrent chromosomal abnormalities and the Philadelphia chromosome in acute and chronic myeloid leukemias: report of five cases and review of literature

Case

Morphology

Flow cytometry

1

Significantly active bone marrow hyperplasia. Progenitor and immature cells accounted for 82.5%. Cell bodies were non-uniform in size and round or nearly round in shape, with few azurophilic granules and visible Auer rods. Nuclei were irregular with indentations, folds, kidney shapes, and so on. Nuclear chromatin appeared as uniformly distributed fine granules, with 2–5 nucleoli.

91.81% of cells were abnormal;

(+): CD33, CD13, CD117, CD123;

Partial expression: HLA-DR;

dim: MPO, CD64;(−): CD34, CD38, CD7, CD5, CD11b, CD56, CD19, CD20, CD10, CD4, CD14, CD36, cCD3, cCD79a

2

Significantly active bone marrow hyperplasia with increased proportion of granulocytes. The majority of cells were granulocyte progenitors, accounting for 70.5%. Cell bodies were non-uniform in size, round or nearly round in shape, had deep margins, and few azurophilic granules. Nuclei were irregular with indentations and folds. Nuclear chromatin appeared as uniformly distributed fine granules, with 2–4 nucleoli.

83.5% of cells were abnormal;

(+): CD33, CD13, CD117, CD123;

Partial expression: HLA-DR,CD34;

dim: MPO, CD64;

(−): CD38, CD7, CD5, CD11b, CD56, CD19, CD20, CD10, CD4, CD14, CD36, cCD3, cCD79a

3

Extremely active bone marrow hyperplasia. Progenitor and immature cells accounted for 84%. Cell bodies were non-uniform in size and round or nearly round in shape, with few azurophilic granules. Nuclei were irregular with indentations, folds, and so on. Nuclear chromatin appeared as uniformly distributed fine granules, with 2–4 nucleoli.

73.91% of cells were abnormal

(+): CD33, CD13, CD123;

Partial expression: CD34, CD117, HLA-DR

dim: MPO, CD38;

(−): CD7, CD5, CD11b, CD56, CD19, CD20, CD64, CD10, CD4, CD14, CD36, cCD3, cCD79a

4

Significantly active bone marrow hyperplasia. Progenitor and immature cells accounted for 46%. Cell bodies were non-uniform in size and round or nearly round in shape, and had small amounts of cytoplasm. Purple-red granules were visible in some cells. Most nuclei were distorted and folded, with 1–4 nucleoli, some of which were unclear.

28.8% of cells were abnormal;

(+): CD117,HLA-DR, CD13, CD33, CD34, CD38, CD15, CD64, CD11c, MPO;

(−): CD7, CD5, CD56, CD19, CD20, CD10, CD4, CD14, CD36, cCD3, cCD79a

5

Significantly active bone marrow hyperplasia with increased proportion of granulocytes. Granulocyte progenitors accounted for 4%, of which the majority were early, middle, late, and rod-shaped. Lobulated neutrophils and eosinophils were occasionally found. Basophils were not found.

Low proportion of myeloid progenitor cells on flow cytometry, accounting for 1.57% of nucleated cells. CD117 expression was lost in some cells. Abnormal expression of CD56 and CD13/CD11b differentiation antigens in some granulocytes. Eosinophils were found.