Fig. 7

FISH with the DNA-probe to individual marker chromomere on GGA1 q-arm after immunodetection of H4Ac and H3K9me3. a – immunodetection of H4Ac on GGA1. f – immunodetection of H3K9me3 on the fragment of GGA1 q-arm. b, g – DAPI staining. c, h – FISH with the DNA probe #16–16 to individual marker chromomere of GGA1. d, i – merged a-c and f-h images, correspondingly (immunostaining – red, DAPI – blue, FISH signals – green). a′-d′, f′-i′ – enlarged areas of panels a-d and f-i framed on d and f. Arrows indicate FISH signals. Scale bars: a-d, f-i – 20 μm; a′-d′, f′-i′ – 10 μm. e, j – maps of H4Ac and H3K9me3 distribution on GGA1, correspondingly. DAPI-positive chromomeres – white circles, DAPI-negative chromomeres – black circles, DAPI-positive chromomeres enriched with H4Ac or H3K9me3 – green circles, DAPI-negative chromomeres enriched with H4Ac or H3K9me3 – orange circles. Arrows indicate position of the chromomere hybridized with the DNA probe #16–16, dashed line (j) indicates border of the GGA1 fragment (f - i). CEN – centromere position, TBL – telomere bow-like loops