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Fig. 3 | Molecular Cytogenetics

Fig. 3

From: Mapping epigenetic modifications on chicken lampbrush chromosomes

Fig. 3

Distribution of H4Ac, H3K9me3 and 5mC on chicken lampbrush chromosome 3 (GGA3). Immunostaining of GGA3 with antibodies against H4Ac (a), H3K9me3 (b) and 5mC (c). a′, b′ – DAPI staining of corresponding chromosomes. a′′, b′′, c′ – merged images of corresponding chromosomes (immunostaining – red, DAPI – blue (a′′, b′′) or cyan (c′). Scale bars – 20 μm. a′′′, b′′′, c′′ – maps of corresponding epigenetic modifications distribution. Intensity of immunostaining and DAPI-staining pattern are indicated by colors as on Fig. 1a′′′, b′′′, c′′. Numbers and lines (1–8) indicate chromomere clusters or chromosome regions described in the Results section. CEN – centromere position, TBL – telomere bow-like loops, LL32 – lumpy loop 32

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