Skip to main content
Fig. 2 | Molecular Cytogenetics

Fig. 2

From: Mapping epigenetic modifications on chicken lampbrush chromosomes

Fig. 2

Distribution of H4Ac, H3K9me3 and 5mC on chicken lampbrush chromosome 2 (GGA2). Immunostaining of GGA2 with antibodies against H4Ac (a), H3K9me3 (b) and 5mC (c). a′, b′ – DAPI staining of corresponding chromosomes. a′′, b′′, c′ – merged images of corresponding chromosomes (immunostaining – red, DAPI – blue (a′′, b′′) or cyan (c′)). Scale bars – 20 μm. a′′′, b′′′, c′′ – maps of corresponding epigenetic markers distribution. Intensity of immunostaining and DAPI-staining pattern are indicated by colors as on Fig. 1a′′′, b′′′, c′′. Numbers and lines (1–5) indicate chromomere clusters or chromosome regions described in the Results section. CEN – centromere position, TBL – telomere bow-like loops, SM – spaghetti marker, LL2 – lumpy loop 2. Staining of the chromomeres with anti-H4Ac and anti-H3K9me3 at the loci of SM formation were not indicated on maps since SM cross-reacts with rabbit serum

Back to article page