Fig. 1

Distribution of H4Ac, H3K9me3 and 5mC on chicken lampbrush chromosome 1 (GGA1). Immunostaining of GGA1 with antibodies against H4Ac (a), H3K9me3 (b) and 5mC (c). a′, b′ – DAPI staining of corresponding chromosomes. a′′, b′′, c′ – merged images of corresponding chromosomes (immunostaining – red, DAPI – blue (a′′, b′′) or cyan (c′)). Scale bars – 20 μm. a′′′, b′′′, c′′ – maps of corresponding epigenetic modifications distribution. DAPI-positive chromomeres – white circles, DAPI-negative chromomeres – black circles, DAPI-positive chromomeres enriched with an epigenetic marker – green circles; DAPI-negative chromomeres enriched with an epigenetic marker – orange circles. Numbers (1-7) indicate chromomere clusters or chromosome regions described in the Results section. CEN – centromere position, TBL – telomere bow-like loops