Table 2 Categories for digital FISH image analysis per probe type

Normal (2F)

Normal (2R, 2G)

Normal (2R, 2G)

One pair (1F)

One pair (1R, 1G)

Ratio - Not amplified (ratio 1, ≥3R, ≥3G)

Breakapart (1F, 1R, 1G)

Fusion (1(− 2)F)

Ratio - Gain^a

Single red (1F, 1R)

Single red (2G, 1R)

Ratio - Amplified^a

Single green (1F, 1G)

Single green (2R, 1G)

Copy number - Not amplified^a

Extra red (2F, ≥1R)

Extra red (2G, ≥3R)

Copy number - Gain^a

Extra green (2F, ≥1G)

Extra green (2R, ≥3G)

Copy number - Amplified^a

Gain (3 - 8F)

Gain (3 – 8R, 3 – 8G)

Amplification (≥8F)

Amplification (≥8R, ≥8G)