Fig. 4
From: An improved method for inducing prometaphase chromosomes in plants
Prometaphase and pachytene chromosomes of Abelia × grandiflora (a and b) and Cucumis melo (c and d). FISH detection of 45S rDNA in somatic metaphase (a1-a3) and pachytene (b1-b3) chromosomes of Abelia × grandiflora. Arrowhead shows a weak signal of 45S rDNA. Asterisk shows overlapped chromosomes, and inset shows the arrangement of two pairs of chromosomes with 45S rDNA signals (a3). FISH detection of melon centromeres in pachytene cells (c1-c3) and prometaphase cells (d1-d3). Somatic metaphase and pachytene chromosome of Abelia × grandiflora (a1 and b1) and melon pachytene chromosomes (c1) were acquired using a combination of MC II and ADI methods. Melon prometaphase chromosome spread (d1) was obtained using the germinated root with the MC II method. Red signals in a2 and b2 are 45S rDNA. Green signals in c2 depict Cmcent labeled with digoxigenin. Green signals in d2 are Cmcent labeled with biotin. Scale bars = 10 μm