Fig. 5

a, b Karyotype-arrays of five cells (K1 to K5) of hybridoma Hyb cl-12 ab + (a) and hybridoma Hyb cl-9 ab + (b). As described in Fig. 4 karyotype-arrays reveal the clonality of cancer-specific chromosomes based on the percentage of cells with chromosomes that form parallel lines and thus have identical copy numbers. The arrays of hybridoma Hyb CN-13 ab + and of hybridoma Hyb cl-9 ab + shared highly clonal copies of all 31 myeloma-specific, abnormal marker chromosomes described in Fig. 4a and Table 2. They also share highly clonal copies of all normal mouse chromosomes from the parental B-cell, although at individually distinct copy numbers. Based on the shared clonal myeloma-specific and clonal normal mouse chromosomes shown in Table 2, the two hybridomas are individually distinct clonal subspecies of the myeloma and normal B-cell. The presence of complete sets of normal mouse chromosomes in both hybridomas is consistent with their production of mouse anti-bodies